气相色谱-质谱法检测人血浆中的盐酸丙哌维林及其氮氧化物

Determination of propiverine and its N-oxide metababolites in human plasma by means of gas chromatography mass spectrometry

目的 建立简便、灵敏的GC MS定量分析方法 ,以测定人血浆中的盐酸丙哌维林 (P4)及其主要代谢产物丙哌维林氮氧化物 (P4NO)。方法 血浆经碱化 (pH9) ,氯仿提取。以地西泮为内标 ,GC MS选择离子检测。结果 盐酸丙哌维林浓度在 6 .2 5～ 40 0ng·ml- 1 内具有良好的线性关系 ,最低检出浓度约为 3.13ng·ml- 1 。血浆中丙哌维林的回收率为 81.7%～ 87.5 % ,日内RSD <7.7% ,日间RSD <10 .4%。结论 本法适用于盐酸丙哌维林的生物利用度研究和血药浓度监测。

OBJECTIVE To develop a simple and sensitive gas chromatography mass spectrometry method for the determination of propiverine hydrochloride (P4) and its N oxide metabolites.METHODS The analytes were alkalized (pH 9) and extracted from plasma into chloroform.It was analysed by the selected ion monitoring method.Diazepam was used as the internal standard.RESULTS The linear range of the calibration curves for P4 was 6.25～400 ng·ml -1 and the limit of quantitation was about 3.13 ng·ml -1 .The recoveries of P4 were 81.7% ～87.5%.The RSD within day and day to day were less than 7.7% and 10.4%,respectively.CONCLUSION The method appears to be appropriate for the bioavalilability studies of propirerine.