摘要
This study developed an efficient in vitro cultivation and propagation sys- tem for an endangered species Kolkwitzia amabilis using nodal segments as ex- plants. Multiple shoots were induced through axillary bud formation. The highest fre- quency of multiple shoot induction was achieved when the nodal segment explants were incubated in Murashige and Skoog （MS） medium supplemented with 4.44 pM 6-benzyladenine （6-BA） in combination with 0.54 μM a-naphthaleneacetic acid （NAA）, followed by treatment with 4.44 μM 6-BA in combination with 0.27 μM NAA. Shoot multiplication could be induced in MS medium supplemented with stand-alone 6-BA or 6-BA in combination with indole-3-acetic acid （1.71 μM） or NAA （0.27 or 0.54 μM）, with 6-BA and either compound, exhibiting a stronger effect on shoot multiplication. The optimum combination of plant growth regulators for shoot multiplication was 4.44 μM 6-BA with 0.27 μM NAA. The maximum rooting percentage was obtained in a half-strength MS medium supplemented with indole-3-butyric acid alone and in com- bination with NAA and 2,4-dichlorophenoxyacetic acid, but the best combination of plant growth regulators for rooting was 1.48 μM indole-3-butyric acid with 1.08 μM NAA and 0.05 μM 2,4-dichlorophenoxyacetic acid. The rooted shoots were trans- ferred to a greenhouse with a success rate of 100%.
该研究采用珍稀植物猬实的茎节段为外植体,通过丛生芽诱导建立了高效的离体培养及快速繁殖体系。丛生芽诱导的最适培养基是MS+4.44μM6-BA+0.54μMNAA,其次是MS+4.44μM6-BA+0.27μMNAA;在MS基本培养基中添加6-BA或者6-BA与IAA(1.71μM)或NAA(0.27,0.54μM)结合能促进丛生芽的增值,增值系数为3.4-8.2,增值系数最大的培养基是MS+4.44μM6-BA+0.27μMNAA;对于生根,1/2MS培养基中添加IBA或者IBA结合2,4-D和NAA能够获得100%生根率,而最适合的浓度组合为1/2MS+1.48μMIBA+1.08μMNAA+0.05μM2,4-D;生根苗移栽温室100%成活。
