Protective Effects of Aqueous Extract of Eucommia ulmiodes Oliver Leaves on Liver Mitochondria

杜仲叶水提物对肝线粒体的保护作用(英文)

[Objective] This study aimed to investigate the protective effects of aqueous extract of Eucommia ulmiodes Oliver leaves （AEO） on liver mitochondrial injury induced by free radicals. [Method] MDA content was determined by thiobarbituric acid colorimetric method; mitochondrial swelling degree was determined by spectrophotometry; the superoxide anion radical scavenging ability was determined using reduced coenzyme I-tetrazolium-phenazine methosulfate as the superoxide anion generation system. Mice were hypodermically injected in the back and neck with D- galactose, after 50 d, the effects of AEO on the activities of superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px） and anti-hydroxyl radicals in mouse liver were determined using kits. [Result] AEO can efficiently reduce the liver injury and inhibit mitochondrial swelling induced by Fe2＋-L-Cys, which can also scavenge superoxide anion and improve the activities of antioxidant enzymes. [Conclusion] This study provided scientific basis for the development and application of AEO resources.

[目的]研究杜仲叶水提物对自由基所致的肝线粒体损伤的保护作用。[方法]采用硫代巴比妥酸比色法测定丙二醛含量、分光光度法测线粒体的肿胀度,并以还原型辅酶I-氮蓝四唑-吩嗪硫酸甲酯为超氧阴离子生成系统测定对超氧阴离子的清除能力。小鼠背部注射D-半乳糖50d后,利用试剂盒测定杜仲叶水提物对小鼠肝脏超氧化物歧化酶、谷胱甘肽过氧化物酶和抗羟自由基活性的影响。[结果]杜仲叶水提物可减轻肝脏的损伤,抑制线粒体肿胀,具有清除超氧阴离子,增强抗氧化酶的活性。[结论]该研究为杜仲叶资源的开发与应用提供了科学依据。