Kruppel样因子在人食管癌细胞中的作用研究

Effects of Kruppel like factor on human esophageal cancer cells

目的探讨Kruppel样因子(KLF4)对食管癌细胞生长、侵袭及迁移等生物学行为的影响,揭示其与食管癌发生发展的关系。方法检测人食管癌细胞株KYSE140、KYSE150、EC109及EC9706及食管永生化细胞NE3中KLF4的表达,通过siRNA的方法将KLF4高表达的KYSE140细胞敲降KLF4基因,通过MTT试验、软琼脂集落形成实验、Transwell小室侵袭实验检测该细胞生物学行为的变化。结果与转染control siRNA的细胞相比,转染KLF4-siRNA1、KLF4-siRNA2的KYSE140细胞生长速度上升,细胞穿过Transwell微孔膜的细胞数量增加,并且形成的集落明显增加,差异均有统计学意义(P<0.05)。结论 KLF4在人食管癌细胞的增殖、侵袭和迁移过程中起着负性调节的作用,可能成为早期诊断和判断食管癌预后的分子标志物以及临床治疗的分子靶点。

Aim To investigate the effect of Kruppel like factor 4 （ KLF4 ） on esophageal cancer cell growth, invasion and migration, and reveal its relationship with the occurrence and development of human esophageal cancer. Methods Expression of KLF4 was detected in the esophageal cancer cell lines, including KYSE140, KYSE150, EC109 and EC9706, and immortalized esophageal cell NE3. KLF4 highly expressed KYSE140 cell was chosen to demonstrate the biological behavior changes after silencing the KLF4 with siRNA transfection by MTT test, colony formation test, and Transwell cell invasion assay. Results KLF4-siRNA1 and KLF4-siRNA2 transfected KYSE140 cells accelerated the proliferation and amount of Ttranswell invasion ceils and the colony formation increased. There was statistically significant difference between the siRNA transfected and control cells （ P 〈 0.05 ）. Conclusion KLF4 negatively regulated the proliferation, invasion and migration of esophageal cancer cells, and it may be served as an emerging molecular biomarker for early diagnosis and therapeutic target for esophageal cancer.