真菌对龙胆苦苷的生物转化研究

Biotransformation of Gentiopicroside by Fungus

目的:采用非恒定温度的培养方法,研究5种真菌对底物龙胆苦苷的生物转化。方法:用薄层色谱法(TLC)以及高效液相色谱法(HPLC)检测转化后生成的产物,并用气相色谱-质谱联用仪(GC-MS)定性分析。结果:该研究证明黑曲霉、米根霉、少根根霉、酿酒酵母YS58和毕赤酵母GS115均能转化龙胆苦苷。其中通过GC-MS定性分析,黑曲霉、米根霉、少根根霉以及毕赤酵母GS115的转化产物之一确定为龙胆酸(2,5-dihydroxybenzoic acid)。结论:与龙胆苦苷相比,转化产物龙胆酸同样具有抗炎的药理作用,并且无毒副作用。

Objective：Using 5 fungus culture by non-constant temperature to transform gentiopicroside. Methods：Thin layer chromatography（TLC） and high performance liquid chromatography（HPLC） are applied to detect the transformation products and gas chromatography-mass spectrometry（GC-MS） is used for qualitative analysis. Results：Through the TLC map and HPLC chromatogram, the results shows that Aspergillus niger, Rhizopus oryzae, Rhizopus arrizus, Saccharomyces cerevisiae YS58 and Pichia pastoris GSll5 have the ability of transforming gentiopicroside. Among them, through the qualitative analysis of GC-MS, one of the transformation products of Aspergillus niger, Rhizopus oryzae, Rhizopus arrizus and Pichia pastoris GS 115 is 2,5-dihydroxybenzoic acid. Conclusion ： Compared with gentiopicroside, the transformation products 2,5-dihydroxybenzoic acid also has the anti-inflammatory pharmacological effects, and toxic side effects.