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siRNA沉默PELP1基因对结肠癌Lovo恶性生物学行为影响的实验研究 被引量：2

Influence of PELP1 downregulation by siRNA in colon cancer cell line Lovo
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摘要 目的:明确雌激素受体共调节因子脯氨酸,谷氨酸,亮氨酸富集蛋白1(PELP1)在结肠癌Lovo中的作用及其机制。方法:使用siRNA沉默结肠癌Lovo中的PELP1基因,并观察沉默前后结肠癌Lovo恶性生物学行为的变化。结果:PELP1的沉默效率可达80%,沉默后Lovo的增殖能力、克隆形成能力、迁移和侵袭能力均下降。结论:在结肠癌Lovo中沉默PELP1可以降低Lovo的恶性程度,提示PELP1在Lovo中起癌基因的作用。 Objeetive： To identify the role of PELP1 in colon cancer cell line Lovo and its mecha- nism. Methods： The expression of PELP1 was silenced by siRNA in Lovo and the change of Lovo re- lated to malignant behaviour was watched. Results： The silent efficience of siRNA for PELP1 reached 80%.After PELP1 being silent,proliferation ability, colony formation ability, migratory and invasive ability of Lovo decreased. Conclusion： PELP1 acts as an oncogene in colon cancer cell line Lovo.
作者 王伟 武倩 司马学琴 杨琴 刘复兴 宁志丰
机构地区 咸宁市中心医院普外科 湖北科技学院基础医学院解剖学教研室 湖北科技学院基础医学院病理学教研室
出处 《中国现代普通外科进展》 CAS 2013年第4期253-256,共4页 Chinese Journal of Current Advances in General Surgery
基金 湖北省教育厅B类指导性项目(B20112806) 湖北科技学院博士启动基金(BK0908)
关键词 结肠肿瘤·脯氨酸 谷氨酸 亮氨酸富集蛋白1·siRNA Colon neoplasms. Proline, -glutamic acid, -leucine rich protein 1 .siRNA
分类号 R735.3 [医药卫生—肿瘤]
  • 相关文献

参考文献15

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二级参考文献8

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同被引文献27

  • 1王斌,宋燕.骨桥蛋白在结肠癌组织中的表达及血清水平检测[J].吉林大学学报（医学版）,2007,33(2):324-326. 被引量：4
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引证文献2

二级引证文献8

中国现代普通外科进展
2013年 第4期

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