c-Met与胆石相关性肝内胆管癌的相关性研究

c-Met expression in intrahepatic cholagiocarcinoma tissues and its correlation with clinicopathological characteristics

目的:探讨c-Met的表达与胆石症合并肝内胆管癌患者临床病理特点的相关性。方法:收集甲醛固定、石蜡包埋的35例胆石症合并肝内胆管癌及配对的35例癌旁胆管组织、20例正常肝脏胆管组织,所有病例均经病理证实,同时收集胆石症合并肝内胆管癌患者的临床资料。采用免疫组织化学的方法检测组织中c-Met的表达情况。结果:c-Met阳性表达主要以癌细胞胞膜及胞质呈现棕黄色颗粒状染色。在肝内胆管癌组织、癌旁胆管组织和正常肝内胆管组织中的表达阳性率分别为68.57%(24/35)、77.14%(27/35)、10.00%(2/20),肝内胆管癌组织和癌旁胆管组织的表达均高于正常肝内胆管组织(P<0.05)。c-Met的表达与患者是否存在淋巴结转移和肿瘤分化程度有明显相关性(P<0.05)。结论:c-Met可以用来早期诊断,评估预后,并作为判断胆石相关性肝内胆管癌恶性程度的参考。

Objective： To detect the expression of c-Met in intrahepatic cholagiocarcinoma tissues and paired tissues,and to investigate the association between c-Met and clinicopathological parameters of intrahepatic cholagiocarcinoma patients. Methods： c-Met were analysed in 35 cases of intrahepatic changiocarcinoma and paired non-cancerous tissues by immunohistochemical method. Patients＇ clinical parameters were collected meanwhile. Results： c-Met positive signals showed brown-yellow granules in the membrane. The positive rates of c-Met expression were 68.57% （24/35） in intrahepatic cholangiocarcinoma 77.14% （27/35） in paired tissues 10.00% （2/20） in normal intrahepatic bile duct respectively. The positive expression of c-Met protein was signifi- cantly corresponding to lymph node metastasis and histological grade. Conelusion： The expres- sion of c-Met protein may play an important role with intrahepatic cholagiocarcinoma. Expression of c-Met might be associated with aggressive tumor phenotype of invasive intrahepatic cholagiocarci- noma. The expression of c-Met may be a useful prognostic indicator.