限制性应激对1型糖尿病模型大鼠的影响

Restraint stress effects in type 1 diabetes rats

背景:限制性应激为构建心理应激的一种方法。目的:建立合适的糖尿病及限制性应激模型,分析限制性应激与1型糖尿病的关系。方法:选取48只雄性SD大鼠,在腹腔内注射链脲佐菌素构建1型糖尿病大鼠模型。建模成功后将其随机分为2组,实验组施加限制性应激,对照组不施加。于施加限制性应激后的1,2,3和4周分别处死实验组和对照组的大鼠各6只,检测相关应激标识物:促肾上腺皮质激素、皮质酮以及胰高血糖素在血清中的浓度。并定期进行空腹血糖测试。结果与结论:实验组大鼠在施加限制性应激1周后血糖及胰高血糖素增高显著高于对照组(P<0.05或P<0.001);血清中促肾上腺皮质激素,皮质酮等应急标识物水平显著高于对照组(P<0.05或P<0.01)。说明限制性应激可以升高糖尿病大鼠的血糖水平。

BACKGROUND： A way to build psychological stress is restraint stress. OBJECTIVE： To study the relationship of restraint stress and type 1 diabetes METHODS： Forty-eight male Sprague-Dawley rats underwent intraperitoneal injection of streptozotocin to rat models of type 1 diabetes mellitus. After the successful modeling, rats were randomly divided into two groups： stress group （the rats were exerted with restraint stress） and control group （no intervention）. Six rats from either group were executed at 1, 2, 3 and 4 weeks after exerting stress, and the related stress markers were detected, including the levels of adrenal cortical hormone, corticosterone and glucagon in the serum. Fasting blood glucose was measured regularly in rats. RESULTS AND CONCLUSION： In the stress group, the levels of glucose and glucagon, after a week of the stress, were higher than those in the control group （P 〈 0.05 or P 〈 0.001）. Simultaneously, the levels of adrenal cortical hormone and corticosterone in serum were higher in the stress group compared with the control group （P 〈 0.05 or P 〈 0.001 ）. These indicate that restraint stress can raise the blood sugar level of diabetic rats.