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帕金森大鼠消化道组织中蛋白质NHERF家族和CAL的表达变化 被引量：3

Change of NHERF family protein and CAL protein in Parkinson's rats gastrointestinal tissue
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摘要 目的研究NHERF家族蛋白和CAL蛋白在帕金森病(Parkinson’s disease,PD)消化系统中的表达,探讨帕金森病消化系统功能紊乱的可能机制。方法用6-羟多巴(6-hydroxydopamine,6-OHDA)损毁大鼠中枢黑质多巴胺能神经元,建立帕金森大鼠模型。采用蛋白免疫印迹法(Western blotting),检测NHERF1﹑NHERF2﹑NHERF3﹑NHERF4以及CAL在6-OHDA处理模型大鼠胃体﹑十二指肠﹑结肠中表达的情况。结果成功造模后,利用Western blotting的方法,发现帕金森模型大鼠胃体PDZK1和CAL蛋白表达水平上调,帕金森模型大鼠十二指肠NHERF1、NHERF2、PDZK1表达水平高于正常组,以及帕金森模型大鼠结肠NHERF1、PDZK1、CAL表达水平也高于正常组。结论帕金森大鼠消化道组织中NHERF家族和CAL蛋白表达的上调,为研究帕金森患者胃肠道功能紊乱方面的机制提供线索。 Objective To study the expression of NHERF family protein and CAL protein in the digestive system of Parkinson＇ s disease, and investigate the mechanism of the dysfunction of digestive system in Parkinson＇s disease. Methods Snbstantia nigra dopaminergic neurons of the SD rats were damaged by injecting 6-hydroxydopamine （6-OHDA） in the brain substantia nigra. Western blotting analysis for the expression of NHERF family protein and CAL protein in the gastry, duodenum and colon of the control and 6- OHDA treated rats were performed. Results The Western blotting results showed that the protein level of PDZK1 and CAL in the gastric corpus was enhanced in 6-OHDA treated rats, the protein level of NHERF1, NHERF2 and PDZK1 in the duodenum was increased in 6- OHDA treated rats, the protein level of NHERF1, PDZK1 and CAL in the colon was enhanced in 6-OHDA treated rats. Conclusion The upregulation of NHERF family protein and CAL protein may contribute to the dysfunction of digestive system in 6-OHDA treated rats . These results provide a strong evidence for further studying the mechanisms of digestive system dysfunction in Parkinson＇ s disease.
作者 史小翠 冯小燕 宋然 杨龙燕 贺俊崎
机构地区 首都医科大学基础医学院生物化学与分子生物学系 首都医科大学基础医学院生理学系
出处 《首都医科大学学报》 CAS 2013年第2期232-238,共7页 Journal of Capital Medical University
基金 国家重点基础研究发展计划(973计划)项目(2010CB94480101) 国家自然科学基金(81272887)~~
关键词 NHERF家族蛋白 CAL 6-羟多巴处理模型大鼠 胃 十二指肠 结肠 NHERF family protein CAL 6-OHDA treated rats gastry duodenum colon
分类号 R392.11 [医药卫生—免疫学]
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参考文献22

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共引文献9

同被引文献49

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首都医科大学学报
2013年 第2期

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