Toll样受体11～13在肥大细胞上的表达

Expression of Toll-like receptor 11 -13 in mast cells

目的观察Toll样受体(Toll like receptors,TLRs)11～13在小鼠肥大细胞上的表达。方法用反转录聚合酶链式反应(reverse transcription polymerase chain reaction,RT-PCR)、流式细胞术和免疫荧光染色方法,在mRNA和蛋白质水平检测TLR11～TLR13在小鼠骨髓来源的肥大细胞(bone marrow-derived mast cell,BMMC)和小鼠肥大细胞系MC/9的表达。结果 BMMC和MC/9细胞在mRNA和蛋白质水平均能表达TLR11～TLR13。结论发现肥大细胞表达TLR11～TLR13,为进一步研究其生物学功能提供实验依据。

Objective To identify whether murine mast cells express Toll-like receptors 11-13 （TLR11-13）. Methods Reverse transcription polymerase chain reaction（ RT-PCR）, flow cytometry and immunofluorescent staining were used to detect the expression of TLR11-13 in murine bone marrow-derived mast cell（BMMC） and mast cell line MC/9 at the levels of mRNA and protein. Results Both BMMC and MC/9 expressed TLR11-13 at both mRNA and protein levels. Conclusion The expression of TLR11-13 by mast cells has provided clues to future investigating biological functions of these TLRs in mast ceils.