摘要
目的检测热休克转录因子2（HSF2）在溃疡性结肠炎（UC）、克罗恩病、肠结核、肠淋巴瘤、感染性肠炎、白塞病及正常结肠黏膜中的表达情况。方法收集病理科2003年至2011年存档的肠组织标本：UC组38例，克罗恩病组29例，肠结核组31例，肠淋巴瘤组32例，感染性肠炎组32例，白塞病组28例，正常体检组10例。采用免疫组化检测结肠黏膜HSF2的表达情况。采用图像分析技术计数切片的阳性细胞数，取光学显微镜下的平均阳性细胞数作为计数标准。结果UC患者结肠黏膜组织中HSF2的表达（64．64±15．17）明显高于其他6组（克罗恩病组、肠结核组、肠淋巴瘤组、感染性肠炎组、白塞病组、正常体检组）（32．44±5．94、36．93±6．32、36．16±6．55、37．86±7．76、34．90±5．92、35．54±6．76，P〈0．05），而这6组间差异无统计学意义（P〉0．05）。结论HSF2在UC患者结肠黏膜组织的表达较克罗恩病组、肠结核组、肠淋巴瘤组、感染性肠炎组、白塞病组及正常体检组明显增强，提示HSF2与UC关系密切，HSF2可能对UC的发病、诊断和鉴别诊断起重要作用。
Objective To investigate the expression of HSF2 in colonic mucosa of patients with ul- cerative colitis （UC）, Crohn＇s disease （ C D）, intestinal tuberculosis （ ITB）, intestinal lymphoma （ IL）, infectious enteritis, Behcetg disease and normal control. Methods Intestinal tissue samples were retrieved from 2003 -2011 archived specimen at the Department of Pathology, and assigned to UC group （n = 38 ）, CD group （n =29）, ITB group （n =31 ）, IL group （n =32）, infectious enteritis group （n =32） and Beh- cet＇s disease group （n =28）. 10 cases were recruited as normal control group. The expression of HSF2 in colonic mucosa were detected by immunohistochemistry. Positive cells were counted by Image Analysis. Re- suits The expression rate of HSF2 in intestinal mucosa of UC patients （ 64. 64 ±15.17 ） was significantly higher than that of CD （ 32.44± 5.94 ）, ITB （ 36. 93 ±6. 32 ）, IL （ 36. 16 ±6. 55 ）, infectious enteritis （37.86 ±7. 76）, Behcet＇s disease （34. 90 ±5.92） and normal control （35.54 ±6. 76） （P 〈0. 05）, while there was no significant difference among the latter six groups （ P 〉 0. 05 ）. Conclusion HSF2 is closely re- lated with UC, and may play an important role in the pathogenesis, diagnosis and differential diagnosis of UC.
出处
《中华消化内镜杂志》
2013年第5期253-256,共4页
Chinese Journal of Digestive Endoscopy
基金
国家自然科学基金（81160055/H0310）
云南省科技厅-昆明医科大学联合专项（2011FB183）
