N-甲基-D-天门冬氨酸受体拮抗剂MK-801胚胎脊髓移植后大鼠损伤脊髓凋亡的影响(英文)

N-methyl-D-aspartate (NMDA) receptors antagonist MK-801 prevent apoptosis in rats that received fetal spinal cord transplantation after spinal hemisection

[目的]探讨大鼠脊髓损伤后N-甲基-D-天门冬氨酸(NMDA)受体拮抗剂MK-801胚胎脊髓移植后对大鼠脊髓细胞凋亡的影响。[方法]将动物分为大鼠脊髓半切洞损伤后,分为应用胚胎脊髓和MK-801治疗组(A组),大鼠脊髓半切洞损伤应用胚胎脊髓移植组(B组),单纯脊髓半切洞损伤明胶海绵填充组(C组)。手术后1、3、7、14 d对脊髓损伤区进行细胞凋亡的检测(TUNEL),以及Bcl-2蛋白免疫反应表达的测定(免疫组化法)。采用计算机图像分析技术,进行定量分析。[结果]A、B、C三组中均发现凋亡细胞及BCL-2蛋白免疫反应阳性细胞,图像分析发现,细胞凋亡率为:C>B>A,Bcl-2免疫反应阳性细胞表达顺序为:A>B>C。[结论]大鼠脊髓损伤后应用NMDA受体拮抗剂MK-801和胚胎脊髓移植能抑制脊髓损伤后细胞凋亡。

[Objective] Spinal cord injury （SCI） is the main mason for lmraplegia, but the effective therapies to it is very poor. Research shows that the embryo spinal cord transplantation can repair spinal cord injury, but there still exist a large number of neuron apoptosis in spinal cord, that the antagonist MK-801 for N-methyi＇D-aspartate （NMDA） receptors could reduce the cell death by way of decreasing the concentration of EAA and preventing the extracellular calcium ion influx. In the palmer we would investigate the effect of antagonist MK-801 for N-methyl-D-aslmrtate （NMDA） receptors on apoptosis in spinal cord neuron in rats that received fetal spinal cord transplantation （FSC） after spinal hemisection. [ Method ] Experimental rats were divided into three groups： spinal cord hemisection injury combination with transplants of FSC and MK- 801 treatment group （group A）, spinal cord hemisection injury with transplants Of FSC to injured site group （group B）, spinal cord injury and received geffoam pledgets group （ group C）. After operation rats were killed after 1, 3, 7 and 14 days. The apoptosis of spinal slice from the injrued spinal cord were examined by the methods of the terminal deoxynucleotidal trans- ferase-mediated DUTP-biotin nick end labeling （TUNEL） reaction and the expression of Bcl-2 was measured by immunohistochemistry. The positive cells were quantitative analysis by a computer image analysis system. [ Result] The rate of apopto- sis in spinal cord neuron sequence and the strong expression sequence of Bcl-2 protein expression in spinal cord neuron were found in three groups, they were C 〉 B 〉 A （P 〈 0. 005 ）, A 〉 B 〉 C （ P 〈 0. 05 ） respectively. [ Conclusion ] It indicates that antagonist MK-801 for N-methyl-D-aspartate （NMDA） receptors treatment can prevent apoptosis in spinal cord nell- ron of rats that received fetal spinal cord transplantation after spinal hemisection.