不同油酸亚油酸比值花生品种油酸脱氢酶基因的时空表达特征

Temporal and spatial expression of oleate desaturase gene with different O/L values in peanut varieties

以不同油酸/亚油酸比值(O/L)花生品种为材料,利用实时荧光定量PCR技术,对四个不同O/L花生品种不同组织中油酸脱氢酶基因(FAD2)的表达进行相对定量分析。结果表明:FAD2基因在不同O/L基因型中组成型表达,其在根、茎、叶中表达量低,在花中表达量最高,显著高于荚果等其它组织中的表达量,且品种间差异达显著水平;花U606和花U12在花、幼荚、成熟荚果中的表达量呈依次下降趋势;花育17和狮油红4号在花、幼荚、成熟荚果中的表达量则呈现高-低-次高的表达趋势,差异达显著水平。

Four different O/L peanut varieties were selected to analyze the relative quantitative expression of FAD2 gene in the different tissues by real-time fluorescence quantitative PCR technology. Results showed that FAD2 gene in different O/L genotypes presented constitutive expression. The expression of FAD2 gene were lower in the root, stem and leaves tissues. The expression in the flowers was significantly higher than that in pod and other tissues, and the difference among varieties was significant. The expression of HuaU606 and HuaU12 in flowers, young pods and mature pods tissues declined successively, and the expression of Huayu17 and Shiyouhong No. 4 showed high - low - high expression trends, the difference reached significant level.