摘要
本文研究 AFLP技术在大豆上的应用 ,在改进大豆种子 DNA提取方法的基础上 ,比较同位素与银染检测方法的效果 ,筛选适宜于大豆 AFLP分析的酶切和引物组合 ,从而建立了适用于大豆指纹图谱快速鉴定的 AFL P操作程序 ,并对我国野生大豆和栽培大豆代表性材料进行了
In the present paper, improvement of the DNA extraction from dry soybean seed, comparison between the silver staining and isotope labeling methods, and exploration of the endoenzyme and primer combinations suitable for AFLP analysis in soybeans were presented. Based on this, a simple and fast procedure suitable for soybean AFLP analysis was established,and the representative accessions of G.soja and G.max from various ecological areas in China were examined by using the suggested procedure.
出处
《大豆科学》
CAS
CSCD
北大核心
2000年第3期210-217,共8页
Soybean Science
基金
国家"九五"重点科技项目(No.9601401064)
