VPA联合As_2O_3对NB_4细胞VEGF及Survivin表达的影响

EFFECTS OFVPACOMBINEDARSENIC TRIOXIDE ON THE EXPRESSION OFVEGFAND SURVIVIN IN NB4CELLLINES

目的:探讨丙戊酸钠(VPA)联合三氧化二砷(As2O3)对NB4细胞株血管内皮生长因子(VEGF)、凋亡抑制蛋白Survivin表达的影响。方法:NB4细胞分为对照组、As2O3组及As2O3联合VPA组,RT-PCR方法检测NB4细胞VEGF、Survivin mRNA的表达。结果:As2O30.1μmol/L组NB4细胞VEGF mRNA的表达明显高于对照组、Survivin mRNA的表达明显低于对照组(P<0.05);与As2O30.1μmol/L组比较,As2O30.1μmol/L+VPA 1.0mmol/L组NB4细胞VEGF mRNA、Survivin mRNA的表达均明显降低(P<0.05)。结论:VPA联合小剂量As2O3可以降低单独应用As2O3导致的NB4细胞VEGF表达的升高、并进一步降低Survivin的表达,As2O3联合VPA可降低As2O3治疗急性早幼粒细胞白血病时的不良反应。

Objective：To investigate the effects of valproic acid （VPA） combined arsenic trioxide （As2O3） on expression of vascular endothelial growth factor （VEGF） and Survivin in NB4 cells lines.Methods：NB4 cells were divided into control group, As2O3 group and VPA＋As2O3 group. RT-PCR was used to detect the VEGF and Survivin mRNA expression in NB4 cells.Results：VEGF mRNA expression in NB4 cells ofAs2O3 0.1 μmol/L group was obviously higher than that of control group,while Survivin mRNA expression was obviously lower（P〈 0.05）.Compared with As2O3 0.1μmol/L group,the VEGF and Survivin mRNA expression in NB4 cells ofAs203 0.1μmol/L＋VPA 1.0mmol/L group were all obviously decreased （P〈 0.05）. Conclusions： VPA combined small dose As203 can reduce increasing of VEGF expression in NB4 cells induced by application of As2O3 alone, and can fuaher reduce Survivin expression. So, VPA combined As2O3 can reduce the side effects of As2O3 for treating acute promyelocytic leukemia.