用聚合酶链反应检测鸭瘟病毒的研究

Ditection of Duck Plague Virus Using the Polymerase Chain Reaction

根据已发表的鸭瘟病毒基因序列 ,设计并合成了一对引物 ,其扩增跨幅为 60 2 bp。用这对引物对 6株鸭瘟病毒 DNA进行扩增 ,均获得了与设计大小相符的明亮条带 ,为阳性 ,而对其它 6株禽病病原体基因扩增不出任何条带 ,为阴性。敏感试验表明可检测到 1 0 0 fg的鸭瘟病毒

A set of primers,XZ43、XZ44 were designed according to the published sequences of duck plague virus (DPV). A DPV specific 602 base pair DNA product was amplified by these primers from six DPV strains,but not from six other duck pathogenic virus and bacteria.As little as 100fg DPV DNA was detected using gel electrophroesis.