蝙蝠蛾拟青霉菌发酵菌丝体的化学成分研究

Chemical constituents from fermented mycelium of Paecilomyces hepiali

目的对冬虫夏草真菌蝙蝠蛾拟青霉菌Paecilomyces hepiali发酵菌丝体进行化学成分研究。方法利用硅胶柱、凝胶柱、高效液相色谱等技术对蝙蝠蛾拟青霉菌发酵菌粉的95%乙醇提取物进行分离纯化,并通过核磁共振谱、质谱等谱学方法鉴定化合物结构。结果从95%乙醇提取物的醋酸乙酯萃取部分分离得到11个化合物,分别鉴定为(22E,24R)-麦角甾-7,9,22-三烯-3β-醇(1)、(22E,24R)-麦角甾-7,22-二烯-3β,5α,6β-三醇(2)、(22E,24R)-麦角甾-6β-甲氧基-7,22-二烯-3β,5α-二醇(3)、胡萝卜苷(4)、环(亮-色)二肽(5)、环(异亮-色)二肽(6)、环(缬-色)二肽(7)、4-(2-甲酰基-5-羟甲基-1-氢-吡咯-1-基)丁酸(8)、4-(2-甲酰基-5-甲氧基甲基-1-氢-吡咯-1-基)丁酸(9)、5-羟甲基糠酸(10)、琥珀酸(11)。结论化合物1、2、4～9均为首次从拟青霉(瓶梗青霉)属真菌中分离得到。

Objective To investigate the chemical constituents in the fermented mycelia from Paecilomyces hepiali. Methods The chemical constituents were isolated and purified by repeated silica gel column, Sephadex LH-20, and semi-preparative HPLC chromatography, and their structures were identified by spectroscopic analyses （MS and NMR）. Results Eleven known compounds were isolated from the ethyl acetate fraction of 95% ethanol extract and were identified as ergosterol D （1）, cerevisterol （2）, 6-0- methylcerevisterol （3）, daucosterol （4）, cyclo （L-Leu-L-Trp） （5）, cyclo （L-Ile-L-Trp） （6）, cyclo （L-Val-L-Trp） （7）, 4-[formyl-5- （hydroxymethyl）-lH-pyrrol-l-yl] butanoic acid （8）, 4-[formyl-5-（methoxymethyl）-lH-pyrrol-l-yl] butanoic acid （9）, 5-hydroxyl- methylfin＇oic acid （10）, and succinic acid （I1）. Conclusion Compounds 1, 2, and 4--9 are isolated from the fungi of Paecilomyces Bain for the first time.