马钱子生物碱类成分经口给药后在大鼠体内的药动学研究

In vivo pharmacokinetics of strychnos alkaloids in rats after ig administration

目的比较马钱子生物碱类成分(马钱子碱、马钱子总生物碱和马钱子优化总生物碱)ig给药后在大鼠体内的药动学过程。方法大鼠ig给药后取血,以石杉碱甲为内标,HPLC法测定大鼠血浆样品中马钱子碱,DAS 1.0程序拟合房室模型并计算药动学参数。结果马钱子生物碱类成分ig给药后马钱子碱的药动学行为均符合二室模型;与马钱子碱单体给药相比,马钱子总生物碱与马钱子优化总生物碱能显著增加马钱子碱的体内吸收,其他药动学参数无显著差异。结论 ig给以马钱子生物碱类成分后,马钱子其他生物碱类成分对马钱子碱有吸收促进作用。

Objective To evaluate the in vivo pharmacokinetics of strychnos alkaloids [brucine, total alkaloids in Strychni Semen （TASS）, and optimal total alkaloids in Strychni Semen （OTASS）] by ig administration to rats. Methods The rats were ig administered with strychnos alkaloids and their blood samples were taken. Huperzine A was used as an internal standard. The brucine in plasma of rats was detected by HPLC method. The compartment model was fitted and the phannacokinetic parameters were calculated by DAS1.0 program. Results The pharmacokinetic analysis showed that brucine behaved as a two-compartment model in the three solutions after ig administration in rats. Compared with brucine monomer administration, the solution of TASS and OTASS could obviously increase the absorption of brucine in vivo, but other pharmacokinetic parameters had no significant difference. Conclusion After ig administration with strychnos alkaloids, the other alkaloids in Strychni Semen could promote the absorption of brucine.