肉眼血尿对流式尿沉渣仪红细胞检测的干扰及对策

The Influence of Gross Hematuria on Sysmex UF-500

目的:考察肉眼血尿对流式尿沉渣仪sysmex UF-500的红细胞检测是否造成干扰。方法:挑选9份结果正常的健康体检者新鲜尿标本,每份取2管分为A、B 2组分别与低、高2种浓度肉眼血尿间隔放置,用sysmex UF-500以自动进样模式进行检测并记录红细胞结果。然后观察A、B 2组干扰后结果有无假阳性,并对A组干扰前后红细胞结果进行配对t检验。结果:A组干扰后红细胞结果仍在本室正常参考值范围(<30/ul),无假阳性结果的出现,但t=6.70,p=0.0002说明干扰前后差异有统计学意义;B组干扰后红细胞结果有2例超出本室正常参考值范围(>30/ul),即有2例假阳性。结论:肉眼血尿致流式尿沉渣仪红细胞检测结果较真实结果偏高,差异有统计学意义;且随肉眼血尿浓度的增加可致流式尿沉渣红细胞检测假阳性的出现。

Objective：To investigate influence of the gross hematuria on red cell determination of subsequent adjacent specimen. Methods：Choose healthy fresh urine specimens of 9 copies, and gross hematuria to be detected alternately by automatic sampling mode on the Sysmex UF-500 flow cytometer urine sediment analyzer. Results：Group A：difference in red blood cells count before and after jamming has statistics significance t=6.70,P=0.0002;Group B：2 false positive. Conclusion：Interference of gross hematuria on adjacent follow-up specimens erythrocyte detection is obvious;with hematuria concentration increased and even can lead to false positive results.