大鼠脊髓匀浆诱导胎盘充质干细胞向神经细胞分化的实验研究

Experimental Study of Differentiation of Placenta-Derived Mesenchymal Stem Cells into Neural Cells Induced by the Rat's Spinal Cord Homogenate

目的体外培养大鼠胎盘间充质干细胞,大鼠脊髓匀浆上清液诱导胎盘间充质干细胞向神经细胞分化。方法消化大鼠胎盘组织、贴壁培养获得间充质干细胞,大鼠脊髓匀浆上清液诱导胎盘间充质干细胞分化,采用免疫细胞化学的方法对分化的细胞进行鉴定。结果胎盘组织中分离出的间充质干细胞与骨髓间充质干细胞有相似形态和细胞表面标志,并在脊髓匀浆的诱导下可诱导表达神经元和星型胶质细胞标志:神经元特异性烯醇化酶(NSE)、神经微丝(NF)和胶质纤维酸性蛋白(GFAP)。结论胎盘间充质干细胞能分化为神经元样细胞,脊髓匀浆可以作为一种有效的诱导剂将胎盘间充质干细胞诱导分化为神经细胞。

Objective To culture placental-derived mesenchymal stem cells from rat in vitro,and to observe differentiation of the placenta-derived mesenchymal stem cells into neural cells induced by the ratg spinal cord homoge nate. Methods Rat＇s placental tissue was digested and adherent cultured to obtain mesenchymal stem cells,placental derived mesenchymal stem cells were induced by the ratg spinal cord homogenate, and immunocytoehemical methods was used for identification of differentiated cells. Results Placental-derived mesenchymal stem cells and bone mesenchymal stem cells have similar morphology and cell surface markers, and induced by the spinal cord homogenate, can express markers of neurons and astrocytes ： neuron-specific enolase sign （ NSE ）, neurofilament （ NF） and glial fibrillary acidic pro- tein{ GFAP）. Conclusion Placental mesenchymal stem cells can differentiate into neuron-like cells, and spinal cord ho- mogenate can induee the placenta-derived mesenchymal stem cells to differentiate into neural cells.