p53基因突变在胃癌组织中的检测及临床意义

Detection and Clinical Significance of p53 Gene Mutation in Gastric Cancer

目的探讨p53基因突变在胃癌组织中的检测及临床意义。方法收集20例胃癌标本和25例胃良性疾病标本,分别提取其DNA,用PCR法扩增p53基因,基因测序后进行突变分析。结果 20例胃癌患者中,p53基因的突变率为40.0%,25例胃良性疾病中,p53基因的突变率为8.0%(P<0.05)。结论 p53基因突变在胃癌的发生过程中起重要作用,检测该基因的突变对胃癌的早期诊断、治疗及预后有重要临床意义。

Objective To detect p53 gene mutation and evaluate its clinical significance. Methods A total of 20 cases of gastric cancer tissues and 25 cases of benign gastric disease were colleeted,DNA was separately extracted from them. PCR was used to a,nplify p53 gene. After the gene sequencing,mutations were analyzed. Results The mutation rate of p53 in gastric cancer tissues was 40.0% ,and the mutation rate of p53 in the gastric benign disease was 8.0% （ P 〈 0.05 ）. Conclusion p53 gene mutation plays an important role during the process of gastric cancer,which has clinical significance.