结直肠癌中miR-21的表达及其临床病理学意义

Expression and clinicopathologic significance of miR-21 in colorectal carcinoma

目的探讨结直肠癌组织中miR-21的表达及其临床病理学意义。方法采用TaqMan实时定量RT-PCR法检测43例结直肠癌组织及其癌旁正常组织中miR-21的表达,分析miR-21的表达与结直肠癌临床病理因素之间的关系。结果 miR-21在结直肠癌组织中的表达明显高于癌旁正常组织(P<0.05),结直肠癌组织中miR-21的表达与肿瘤病理学分级、淋巴结转移及术后生存率密切相关(P<0.05),与患者性别、年龄、肿瘤部位等无明显相关性(P>0.05)。结论 miR-21在结直肠癌中表达升高,miR-21高表达同结直肠癌病理学分级、淋巴结转移及术后生存率密切相关,miR-21可能在大肠癌的发生、发展过程中发挥重要作用。

Purpose To investigate the miR-21 expression in cancerous tissues from patients with colorectal carcinoma （CRC） and to evaluate its clinicopathologic significance. Methods TaqMan real-time quantitative RT-PCR was used to detect the miR-21 expression in cancerous tissues and normal tissues from 43 patients with colorectal carcinoma and to observe its relationship with clinicopathologic parameters. Results There was a significantly higher expression of miR-21 in CRC tissues in comparison with adjacent normal colon tissues （P 〈 0.05 ）. High miR-21 expression was associated with clinicopathologic features, such as tumor grade, lymph node metasta- sis and patient survival （P 〈 0. 05 ）. However, there was no relationship between miR-21 expression and other clinicopathological pa- rameters including sex, age and tumor site. Conclusion The expression of miR-21 was up-reg＇ulated in colorectal carcinoma. High miR-21 expression correlates with tumor grade, lymph node metastasis and patient survival, which suggests that miR-21 may play an important role in the development and progression of colorectal carcinoma.