Kail／CD82表达水平与神经母细胞瘤转移的关系探讨

The relationship between Kail/CD82 expression and metastasis of neuroblastoma.

目的探讨Kail／CD82表达水平与神经母细胞瘤转移之间的关系。方法选取40例各期神经母细胞瘤石蜡标本，采用RT—PCR检测KailmRNA的表达；采用免疫组化检测Kai1基因的蛋白产物CD82的表达水平，研究其与神经母细胞瘤转移之间的关系。结果RT．PCR监测显示：有淋巴结或远处转移的神经母细胞瘤KailmRNA的相对含量为0．0748±0．0063，而无转移者为0．4576±0．0226，差异有统计学意义（P〈0．05）。免疫组化显示：无转移的神经母细胞瘤CD82的阳性表达率为71．43％；而有淋巴结或远处转移者阳性表达率为31．58％，差异有统计学意义（P〈0．05），有骨髓浸润者与无骨髓浸润者Kail／CD82的表达无统计学意义（P〉0．05）。结论Kail／CD82表达水平可作为判断神经母细胞瘤是否转移的一个潜在标志。

Objetive To investigate the relationship between Kail / CD82 expression and metastasis of neuroblastoma. Methods RT-PCR shall be applied to test the expression of KailmRNA in 40 neuroblastoma paraffin specimens of each period. Kai1/CD82 level was measured by immunohistochemistry method in 40 neu- roblastoma paraffin specimens of each period. Results RT-PCR shows that the relative content of KailmRNA in in the neuroblastoma group with lymph node or distant metastasis is 0.0748± 0.0063, the relative content of KailmRNA in the neuroblastoma group without metastasis is 0.75 ±0.,03, there is statistical significance in the two group difference. The positive rate of Kail/CD82 expression was 71.43% in the neuroblastoma group with- out metastasis and 31.58% in the neuroblastoma group with lymph node or distant metastasis. There was a sig- nificant difference in the Kail/CD82 expression between neuroblastoma groups with and without metastasis. There was no significant differences in the Kai1/CD82 expression between metastasis neuroblastoma groups with and without bone marrow infiltration. Conclusions The expression level of Kail/CD82 can be used as a po- tential predictor of metastasis of neuroblastoma.