慢性粒细胞白血病患者杀伤细胞免疫球蛋白样受体基因多态性的研究

Polymorphism of human killer cell immunoglobulin-like receptor in patients with chronic myelogenous leukemia

目的:了解慢性粒细胞白血病(CML)患者杀伤细胞免疫球蛋白样受体(killer cell immunoglobulin-like receptor,KIR)基因型及单倍型,为探讨KIR在慢性粒细胞白血病中发挥的作用奠定基础。方法:采用PCR-SSP方法对84例慢性粒细胞白血病患者进行KIR基因分型。结果:框架基因2DL4、3DL2、3DL3及3DP1存在于所有个体中,其他较为高频的基因依次为2DL1、2DL3、2DP1及3DL1,表型频率均>80%。CML患者的KIR2DL2和KIR2DS2表型频率较健康人有所降低,但除2DL5B(OR=0.208,P<0.05)外,分析的所有基因在CML患者和健康人间的分布均不具有显著差异。结论:基因KIR2DL5B可能与CML之间存在相关性。

Objective：To investigate the killer immunoglobulin-like receptor （KIR） genotype in patients with chronic myelogenous leukemia （CML）. Methods：The sequence specific primer polymerase chain reaction （SSP-PCR） was employed to identify the KIR genes and pseudogenes in 84 CML patients. Results：The most frequent genotypes were KIR frame genes,including 2DL4,3DL2,3DL3 and 3DP1 （100%）,followed by 2DL1,2DL3,2DP1 and 3DL1 （all above 80%）. The frequencies of 2DL2 and 2DS2 in CML patients were lower than those in the control group,but there were no statistically difference between the two groups except 2DL5B （OR = 0.208,P 〈 0.05）. Conclusion： KIR 2DL5B may be associatied with CML.