摘要
目的:探讨膜型基质金属蛋白酶-1(MT1-MMP/MMP-14)在骨性关节炎(OA)患者膝关节滑膜中的表达和意义。方法:应用免疫组化和Western blot方法检测MT1-MMP蛋白在40例OA与40例非OA患者滑膜中的表达情况。结果:免疫组化结果表明MT1-MMP蛋白在OA与非OA患者滑膜中的表达有显著性差异(χ2=29.46,P<0.01),且阳性表达多定位于滑膜细胞的胞质;Western blot分析结果显示MT1-MMP蛋白在OA患者滑膜(1.042±0.219)中表达显著高于非OA患者滑膜组织(0.256±0.050,F=12.838,P=0.001)。结论:MT1-MMP蛋白与骨性关节炎发生发展相关,可能作为诊断及判断骨性关节炎的一项重要指标。
Objective:To investigate the expression and significance of MT1-MMP in the synovium of knee joint of patients with osteoarthritis. Methods: We detected the expression of MT1-MMP protein in 80 cases of osteoarthritis by using.immunohistochemically and Western blot. Results:The expression of MT1-MMP protein in the synovium of knee joint of patients with osteoarthritis was markedly higher that in the normal synovium (X2=29.46,P 〈 0.01), and the MT1-MMP protein was mainly located in the cytoplasm; The results of Western blot showed that the expression of MT1-MMP protein in the synovium of knee joint of patients with osteoarthritis (1.042 ± 0.219) was significantly higher than that in the nou-osteoarthritis (0.256 ± 0.050,F = 12.838,P = 0.001). Conclusion:There is a close relation between the expression of MT1-MMP protein and OA, it may be the marker of diagnosis and the judgment of osteoarthritis.
出处
《南京医科大学学报(自然科学版)》
CAS
CSCD
北大核心
2013年第4期494-497,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金(30960387)
石河子大学科学技术研究发展计划(ZRKXYB-16)