摘要
目的:探讨乳腺上皮中BRCA1和BRCA2的表达与乳腺浸润性导管癌的临床病理特征的相关性。方法:应用免疫组化S-P法检测BRCA1、BRCA2在33例乳腺浸润性导管癌组和31例乳腺良性病变组的表达情况,并联合ER、PR、CerBb-2的表达,分析它们与乳腺浸润性导管癌患者的年龄、肿块大小、分化程度、淋巴结转移等特征之间的关系。结果:①BRCA1的阳性表达与乳腺浸润性导管癌患者的年龄、分化程度、是否绝经均有显著相关性(P<0.05),与淋巴结转移和肿瘤大小无显著相关性(P>0.05)。BRCA2的阳性表达与乳腺浸润性导管癌患者的年龄、淋巴结转移、肿块大小、是否绝经均有显著相关性(P<0.05),与分化程度无显著相关性(P>0.05)。②BRCA1、BRCA2的表达与CerBb-2的表达均呈显著的负相关(r=-0.355,-0.517,P<0.05)。结论:BRCA1和BRCA2的突变在乳腺浸润性导管癌的发生发展密切相关,主要通过转录途径作用于CerBb-2,BRCA1和BRCA联合CerBb-2可作为评价乳腺癌生物学特性和预后的潜在指标。
The expressions of breast cancer susceptibility genel ( BRCA1 ) and breast cancer susceptibility gene2 (BRCA2 )in breast epithelia are explored. The intrinsic relevance among the expres- sions of BRCA1 and BRCA2, the develop- ment and prognosis in invasive ductal carci- noma of breast are investigated. Methods: The immunohistochemical S- P method wasused to detect the level of BRCA1, BRCAz in 33 cases of breast invasive ductal carci- noma tissue samples and 31 cases of breast benign disease tissue samples. Associating with the expressions of estrogen receptor ( ER), progestogen receptor ( PR), and epi- dermal growth factor receptor2 ( CerBb - 2 ) , an analysis is needed in the relationship be- tween the expressions of ER, PR, CerBb - 2,BRCAI ,BRCA2 and the characters of pa- tients such as the age, the size of tumor, the grade of differentiation, and lymph node me- tastasis. Results:①The positive expression in BRCA1 exhibits an apparent relevance with the characters of patients such as the age, the size of tumor, the grade of differen- tiation, lymph node metastasis, menopausal age( P 〈 0.05 ) ,while it shows little correla- tions to the grade of differentiation ( P 〉 0. 05 ). (~ The expressions of BRCA1 and BRCA2 were negatively correlated to the ex- pression of CerBb - 2 ( r = - 0. 355, or - 0. 517, P 〈 0.05 ). Conclusion : The mutation of BRCAl and BRCA2 takes a key role in the generation of breast invasive ductal carcino- ma,which can be as a reference to detect bi- ological behavior or prognosis in breast car- cinoma.
基金
奎屯市2011年度科技项目(项目编号:201122)