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2种基因分型法在牛结核病流行病学调查中的应用 被引量：1

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摘要 为了探讨间隔区寡核苷酸分型(Spoligotyping)和数目可变串联重复序列结核分枝杆菌散在分布重复单元(VNTR-MIRU)基因分型法用于国内牛分枝杆菌的基因分型,初步了解盐城地区牛分枝杆菌基因型种类、分布以及2种方法在当地应用的可行性。收集盐城和其他地区分离的31株牛分枝杆菌分离株,分别采用Spoligotyping和VNTR-MIRU对这些分离株进行基因分型,比较2种方法的分型效果,评价其在牛分枝杆菌基因分型中的应用。结果:使用Spoligotyping方法,31株牛分枝杆菌呈现出3种基因型;使用VNTR-MIRU方法,31株分离株呈现6种基因型;当2种方法联合使用时,可以分为7种基因型,其中的1个菌株具有独特的基因型,而2株盐城地区分离株显示其与其他地区的牛分枝杆菌同样存在主要的流行型为BCG家族,未发现其基因独特性。将Spoligotyping和VNTR-MIRU联合使用,可有效提高牛结核病的分子流行病学调查和病原学的监测效果。
作者 高峰 于伯华 杨瑞章 王太全 徐帮兴
机构地区 盐城出入境检验检疫局
出处 《畜牧与兽医》 北大核心 2013年第5期64-68,共5页 Animal Husbandry & Veterinary Medicine
基金 江苏检验检疫局科研项目(2010KJ04)
关键词 牛分枝杆菌 SPOLIGOTYPING VNTR—MIRU 基因分型
分类号 S855.2 [农业科学—临床兽医学]
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参考文献6

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共引文献47

同被引文献18

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畜牧与兽医
2013年 第5期

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