摘要
目的探讨胃癌干细胞抗原负载树突细胞(DC)联合细胞因子诱导杀伤细胞(CIK)对胃癌细胞的影响。方法分离人胃癌干细胞,冻融法制备抗原,将胃癌干细胞负载DC-CIK细胞,用流式细胞仪检测胃癌干细胞和DC、CIK细胞表型;将胃癌细胞与DC组、DC-CIK组、胃癌细胞抗原组、负载胃癌干细胞抗原组联合培养,用四甲基偶氮唑蓝法(MTT)检测不同组别对胃癌细胞的杀伤作用。结果经胃癌干细胞抗原刺激的DC细胞,DC表面成熟标志CD83和CD86表达明显增加,CD83和CD86表达率为80.4%,高于DC组、DC-CIK组、胃癌细胞抗原组,差异有统计学意义(P<0.01);MTT检测结果显示:负载胃癌干细胞抗原组、胃癌细胞抗原组、DC-CIK组、DC组对胃癌细胞杀伤率分别为(80.6±0.8)%、(72.3±0.6)%、(58.4±0.2)%和(49.7±0.8)%,差异有统计学意义(P<0.01)。结论胃癌干细胞作为抗原刺激DC细胞,可增强树突状细胞免疫原形表达,促进细胞增殖,提高对胃癌细胞的杀伤作用。
【Objective】 This study is aimed at investigating the killing effect of dendritic cells（DC） and cytokine-induced killer（CIK） cells loaded with gastric cancer stem cell antigens against gastric cancer cells.【Methods】Human gastric cancer stem cells are Isolated and identified.Antigens are made with the freeze-thaw method.We load gastric cancer stem cells to DC-CIK cells and examine the DC cell phenotype with flow cytometry.Methyl thiazolyl tetrazolium（MTT）method was used to examine the killing effect of DC-CIK on gastric cancer cells.【Resluts】Gastric cancer stem cell antigen-stimulated DC cells the DC surface maturity logo CD83 and CD86 expression significantly increased,CD83 and CD86 expression was 80.4%,higher than the DC group,DC-CIK group,gastric cancer cell antigen group,（P＆lt;0.01） differences;MTT test results show that：the destruction rate of gastric cancer cell destruction,load gastric cancer stem cell antigen group,gastric cancer cell antigen group,DC-CIK group,DC group were（80.6±0.8）%,（72.3±0.6）% was statistically significant;（58.4±0.2）% and（49.7±0.8）%（P＆lt;0.01）,the difference was statistically significant.【Conclusion】Stimulating DC cells with gastric cancer stem cells as an antigen can enhance the immunoreaction,promote cell proliferation,and therefore improve killing effect on gastric cancer cells.
出处
《中国医学工程》
2013年第3期1-2,5,共3页
China Medical Engineering
基金
国家自然科学基金资助项目(30672771
81072969)
关键词
胃癌干细胞
树突状细胞
抗原
stomach cancer stem cells
dendritic cells
tumor antigen
