1株蛇足石杉根际铁载体细菌的分离、筛选与鉴定

Isolation,Screening and Identification of Sidero-Carrier Bacteria from Huperzia serrata Rhizosphere

对蛇足石杉根际铁载体细菌进行了分离、筛选与鉴定,以期筛选到潜在高效促生细菌。采用梯度稀释涂平板法从蛇足石杉根际土中分离细菌,再通过CAS检测平板分析,从中筛选出1株产生铁载体能力较强的菌,并结合16S rDNA基因系统发育分析对可产铁载体的蛇足石杉根际细菌初步鉴定。从蛇足石杉根际中筛选到1株产铁载体细菌JSX 389,经鉴定为Lysinibacillus属菌株。

In order to obtain potential highly effective plant growth-promoting bacteria, sidero-carrier bacteria （SCB） were isolated, screened and identified from rhizosphere of Huperzia serrata using gradient dilution method, among them a strong SCB strain was rescreened by using the chrome azurol sulfonate （CAS） agar plate assay. The strain was initially identified combined with phylogenetic analysis according to 16S rDNA. A siderophores-producing strain JSX389 was isolated from H. serrata rhizosphere and identified as a strain of the genus of Lysinibacillus.