摘要
对蛇足石杉根际铁载体细菌进行了分离、筛选与鉴定,以期筛选到潜在高效促生细菌。采用梯度稀释涂平板法从蛇足石杉根际土中分离细菌,再通过CAS检测平板分析,从中筛选出1株产生铁载体能力较强的菌,并结合16S rDNA基因系统发育分析对可产铁载体的蛇足石杉根际细菌初步鉴定。从蛇足石杉根际中筛选到1株产铁载体细菌JSX 389,经鉴定为Lysinibacillus属菌株。
In order to obtain potential highly effective plant growth-promoting bacteria, sidero-carrier bacteria (SCB) were isolated, screened and identified from rhizosphere of Huperzia serrata using gradient dilution method, among them a strong SCB strain was rescreened by using the chrome azurol sulfonate (CAS) agar plate assay. The strain was initially identified combined with phylogenetic analysis according to 16S rDNA. A siderophores-producing strain JSX389 was isolated from H. serrata rhizosphere and identified as a strain of the genus of Lysinibacillus.
出处
《微生物学杂志》
CAS
CSCD
2013年第2期63-67,共5页
Journal of Microbiology
基金
国家自然科学基金(31260081)
植物资源保护与利用湖南省高校重点实验室开放基金(JSK200904)
吉首大学校级科研项目资助(Jdy12038)