纳米金免疫层析法定量检测尿RBP

Gold nanoparticle immunochromatographic assay for quantitative detection of urinary RBP

应用纳米金免疫层析技术(双抗夹心法)和柠檬酸三钠还原法建立一种对尿RBP的快速定量检测方法,并在此基础上研制出快速检测试纸条.该试纸条用纳米金免疫层析定量分析仪可在15 min内实现RBP定量检测,检测限为150μg/L(相当于150 ng/mL),检测范围为150～5000μg/L.与尿微量白蛋白(ALB)、转铁蛋白(TRF)、β2-微球蛋白(β2-MG)、尿纤维连接蛋白(FN)、溶菌酶(LZM)等肾脏疾病标志物无交叉反应.该方法特异性强、检测灵敏度高、范围广、简便快捷,在近端肾小管的损伤、糖尿病肾病等肾脏疾病的早期诊断及过程监控中具有较为广泛的临床应用.

A rapid quantitative detection of urinary RBP was established by using nano-gold immunochromatography （ sandwich method） and trisodium citrate reduction method and a rapid immunochromatographic test strip was developed. Theimmunochrom- atographic test strip can quantitatively detect RBP within 15 minutes. The detection limit was 150ng/mL and detection range was from 150 to 5000 ng/mL. There were no cross-reactions with others kidney disease markers, such as urinary albumin （ALB）, transferrin protein （ TRF）, ~2-microglobulin （ ~2-MG）, urinary fiber connecting protein （ FN）, and lysozyme （LZM）. The re- suits indicate that it is a quick and simple method with strong specificity,high sensitivity, and wide detection range. The rapid de- tection method will have extensive clinical applications in the early diagnosis of proximal tubular damage, kidney disease, diabetic nephropathy, and process monitoring.