陆地棉trihelix转录因子基因响应非生物胁迫表达谱分析

Expression Profiling of Cotton (Gossypium hirsutum L.) Trihelix Genes Responsive to Abiotic Stresses

【目的】对陆地棉trihelix转录因子基因在多种胁迫下的应答表达模式进行研究分析,为棉花抗逆相关基因克隆及阐明棉花抗逆分子机制奠定基础。【方法】根据已知24个trihelix转录因子EST序列设计引物,通过RT-PCR和qRT-PCR的方法分析棉花(Gossypium hirsutum L.)GhGT在高盐(200 mmol.L-1NaCl)、干旱、低温(4℃)等非生物胁迫和外源激素ABA(100μmol.L-1)处理下的表达模式。【结果】12个基因响应高盐和干旱胁迫应答,7个基因响应冷胁迫应答,应答ABA处理的基因9个。此外,有3个基因(GhGT2、GhGT18和GhGT23)在4种处理下均有应答。【结论】GhGTs在陆地棉的非生物胁迫适应过程中可能具有重要的作用。

[ Objective ] The objective of this study is to dissect cotton trihelix genes expression alteration responsive to different abiotic stresses, thus providing a foundation for stress-related genes cloning and elucidating the molecular mechanism of cotton stress-resistance. [Method] Semi-quantitative RT-PCR and real-time PCR were employed to reveal the expression profiling of 24 GhGTs under different abiotic stresses （200 mmol.L^-1 NaC1, drought, 4℃） and exogenous hormone （100 Bmol.L^-1ABA） treatments. [Result] The results showed that the expression of 12 GhGT genes were up- and down-regulated by salinity and drought stresses, and that of 7 GhGT genes and 9 GhGT genes were up- and down-regulated by cold and ABA treatments, respectively. Moreover, three genes including GhGT2, GhGT18 and GhGT23 were response to all above treatments. [ Conclusion ] The results suggested that GhGTs may play important roles in upland cotton plant abiotic stress adaption.