晚孕期阴道壁结缔组织Ⅰ型胶原和TIMP-1表达分析

Expressions of type Ⅰ collagen,tissue inhibitors of matrix metalloprot einases-1 in vaginal wall tissue of pregnant women in the third trimester

目的:探讨妊娠晚期妇女阴道壁结缔组织中Ⅰ型胶原蛋白和基质金属蛋白酶组织抑制因子(TIMP-1)mRNA表达改变及二者间的相关性。方法:选取20例无妊娠合并症的足月顺产产妇为晚期妊娠组。另选取未绝经、无盆底功能障碍,因患子宫肌瘤或子宫腺肌症行全子宫切术患者15例为对照组,应用免疫组化和荧光定量PCR法检测标本中Ⅰ型胶原和TIMP-1 mRNA表达水平。结果:晚期妊娠组Ⅰ型胶原蛋白和TIMP-1 mRNA表达水平均显著低于对照组(P<0.05)。结论:妊娠、分娩下调TIMP-1基因转录水平表达,促进胶原蛋白分解增加,盆底支持功能减弱,可能是妊娠晚期盆底松弛及产后盆底功能障碍的重要发病机制之一,也是妊娠、分娩作为PFD的独立危险因素的重要原因。

Aim：To study the expressions of type I collagen, TIMP-1 in the connective tissue of vaginal wall of pregnant women in the third trimester compared to continent controls, and to explore the relationship between them. Methods： Twenty women with no pregnancy complications after vaginal delivery were the late pregnant group, fifteen premenopausal women had gynecology diseases but without relaxation of pelvic supports as control group. All of them accepted our informed consent before their vaginal wall tissue samples were extracted. Real-time fluorescence quantitative PCR（ RT-FQ-PCR）, haematoxylin-eosin（HE） staining and immunohistochemistry were performed to detect the expressions of collagen type I, and TIMP-1 mRNA. Results： The expressions of type I collagen in two groups were 18.68 ± 11.21 and 26. 97 ± 10. 25 and TIMP-1 mRNA in two groups were 4.63 ± 15.39 and 6.00 ±9.26. Significant differences of the expressions of them were observed between the late pregnant group and the control group（ all P 〈 0. 05 ）. Conclusion： In late pregnant group, the low expression of TIMP-1 on gene transcription level in viginal wall tissue could enhance collagen decomposition which might contribute to the development of postpartum pelvic floor dysfunction and explain why pregnancy and childbirth as the independent risk factors of PFD.