不同转移倾向小鼠肝癌瘤株与冰切组织体外作用的实验研究

EXPERIMENTAL STUDY ON INTERACTION OF DIFFERENT METASTATIC POTENTIAL H_(22) CELLS WITH CRYOSTAT SECTIONS OF HOST TISSUES IN VITRO (ABSTRACT)

本实验利用小鼠腹水型肝癌(H_(22))的肺转移倾向亚株(H_(22)SP_(10))与淋巴结转移倾向亚株(H_(22)SL_(10))和小鼠的心、肺、肝、肾、淋巴结冰切组织于体外混合培养,观察了不同转移能力的肿瘤细胞与正常组织间的相互作用。于混合培养24、48h后,计数冰切组织上粘附的肿瘤细胞,肝、肾、淋巴结上粘附的H_(22)细胞多于其它脏器。H_(22)SP_(10)主要粘附于肝、肾、肺,而H_(22)SL_(10)则主要粘附于淋巴结,几乎不粘附于肺组织。混合培养后的生长曲线表明加入的正常组织对三种瘤细胞生长具有不同作用,加入的五种组织对H_(22)生长均有促进作用,加入心、肝、肾组织使H_(22)SP_(10)细胞于培养48h之前呈递增趋势,而加入肺、淋巴结组织使H_(22)SP_(10)细胞生长数低于对照组,加入心脏组织96h后H_(22)SL_(10)细胞数开始高于对照组,而加入其它组织均使H_(22)SL_(10)细胞数低于对照组。该结果表明在活体中呈器官倾向性转移的瘤细胞在体外具有与相应组织特异性粘附的能力,而且这些细胞与不同宿主组织间存在不同相互作用。

The ascites hepatoma (H_(22)) was used to study interaction betweenmetastatic tumor cells and host tissues. Its pulmonary metastaticsubstrain (H_(22)SP_(10)) and lymphatic metastatic substrain was culturedin vitro on cryostat section of host tissues including, lung, heart, kid-ney, liver and lymphatic node. The cell number of tumor cells bindingto cryostat sections was counted after co-culture 24, 48, 72, hours.H_(22) cells which adhered to the normal tissues of lung, kidney andlymphatic node are more than those did to others. H_(22) SP_(10) cell pre-ferentially adhered to the tissues of liver, kidney and luny. In cont-rast, H_(22) SL_(10) cells mainly adhered to tissues of lymphatic node andscarcely to lung. On the other hand, host organ sections had diftenenteffect on the cell number of H_(22) H_(22) SP_(10) and H_(22) SL_(10) respectively.Theresults provide evidence for specific binding of tumor cells to thetarget tissues in vitro and show the interaction between differentmetastatic potential tumor cells and various host tissues in vitro.