5种植物多糖的部分酸水解特征

Characteristics of partial acid hydrolysis in five plant polysaccharides

目的对5种植物多糖的部分酸水解特征及其水解产物的组成进行研究。方法分别采用0.05、0.2和0.5 mol/L三氟乙酸(TFA)对5种植物多糖进行部分酸水解,并研究其水解特征;结合超高效液相色谱及多元统计的方法对其水解产物的组成进行分析。结果 0.05 mol/L TFA水解后截留液中葡萄糖醛酸(X1GlcUA)量、0.05 mol/L TFA水解后透过液中阿拉伯糖(X2Ara)量、0.2 mol/L和0.5 mol/L TFA水解后截留液中阿拉伯糖(Y1Ara、Z1Ara)量、0.2 mol/L TFA水解后透过液中半乳糖醛酸(Y2GalUA)量为麻黄多糖区别其他植物多糖的特征水解片段。结论部分酸水解反应结合色谱分析,超高效液相色谱联合多元统计的方法,分析单糖组成及其量的变化,可以进一步为多糖的质量控制提供有力的依据。

Objective To study the characteristics of partial acid hydrolysis in five different plant polysaccharides and the composition of their hydrolysic products. Methods Polysaccharides were partially hydrolyzed with trifluoroacetic acid （TFA） at different concentration （0.05, 0.2, and 0.5 mol/L）, respectively, and the hydrolytic characteristics were studied by ultra-performance liquid chromatography （UPLC） coupled with multivariate statistricts. Results The characteristic structural segments of ephedra polysaccharide were the contents of glucuronic acid in trapped fluid hydrolyzed with 0.05 mol/L TFA （X1GlcUA）, arabinose in permeation fluid hydrolyzed with 0.05 mol/L TFA （X2Ara）, arabinose in trapped fluid hydrolyzed with 0.2 and 0.5 mol/L TFA （Y1Ara and Z1Ara）, and galacturonic acid in the permeation fluid hydrolyzed with 0.2 mol/L TFA （Y2GalUA）, which could distinguish other plant polysaccharide samples. Conclusion The proposed method is precise and practical for the analysis on monosaccharide compositon in Chinese materia medica by UPLC coupled with multivariate statistics and partial acid hydrolytic reaction, which further provides the basis for the quality control of the otant polysaccharides.