聚乙二醇-6000胁迫对丹参毛状根中丹参酮积累的影响

Effects of PEG-6000 stress on tanshinones accumulation in hairy roots of Salvia miltiorrhiza

目的研究聚乙二醇-6000(polyethylene glycol-6000,PEG-6000)胁迫对丹参毛状根丹参酮类成分积累的影响。方法用发根农杆菌ATCC15834诱导生产丹参毛状根,经20 d悬浮振荡培养后,分别采用1.2%、2.0%、5.5%、10%的PEG-6000处理,并用HPLC法测定PEG-6000处理1周后丹参毛状根中丹参酮I、隐丹参酮、二氢丹参酮I、丹参酮IIA的量。结果PEG-6000能够抑制丹参毛状根的生长,PEG-6000为1.2%、2.0%、5.5%和10%处理条件下,丹参毛状根干质量分别减少为对照组的75.1%、83.0%、76.2%、76.1%;PEG-6000处理能够显著提高丹参毛状根中4种丹参酮的量,2.0%、5.5%的PEG-6000能够显著促进丹参酮I量的提高,1.2%PEG-6000能够显著提高隐丹参酮量的;二氢丹参酮I的量以2.0%PEG-6000处理条件下最高;丹参酮IIA的量以5.5%PEG-6000处理条件下最高。结论 PEG-6000能显著促进丹参毛状根中丹参酮类成分的积累。

Objective To study the effects of polyethylene glycol-6000 （PEG-6000） stress on the accumulation oftanshinones in hairy roots of Salvia miltiorrhiza. Methods Agrobacterium rhizogenes ATCC15834 was used to induce the hairy roots orS. miltiorrhiza. After 20 d suspension culture, the PEG-6000 （1.2%, 2.0%, 5.5%, and 10%, respectively） was added into the suspension cultures and at the same time, the contents of tanshinones （including tanshinone Ⅰ, cryptotanshinone, dihydrotanshinon Ⅰ, and tanshinone ⅡA） were quantified by HPLC on day 7. Results The growth of the hairy roots of S. miltiorrhiza was inhibited by PEG-6000. After PEG-6000 （1.2%, 2.0%, 5.5%, and 10%） treatment, the dry weights of the hairy roots of S. miltiorrhiza were reduced to 75.1%, 83.0%, 76.2%, and 76.1% of the control group, respectively. Addition of PEG-6000 at different levels could significantly increase the yields of four tanshinones in the hairy roots ofS. miltiorrhiza. The yields of tanshinone Ⅰ, cryptotanshinone, dihydrotanshinon Ⅰ, and tanshinone ⅡA were significantly increased by 2.0%-5.5%, 1.2%, 2.0%, and 5.5% PEG-6000, respectively. And the tanshinone ⅡA increased most. Conclusion PEG-6000 could stimulate the accumulation oftanshinones in the hairy roots orS. miltiorrhiza.