草兔种群的遗传多样性与系统进化研究

Genetic Diversity and Phylogenetic Evolution of Lepus capensisPopulation

通过测定江苏滩涂草兔样本的线粒体DNA控制区部分序列,引用中国不同地理种群的草兔mtDNA D-Loop序列作为分析资料,研究了草兔种群的遗传多样性和系统进化关系。结果表明,在10条草兔线粒体DNA控制区566 bp的序列中检测到4个多态性位点,定义了3种单倍型类型。滩涂草兔种群的单倍型多样度和核苷酸多样度分别为0.689和0.003 06,显示出相对较低的遗传多样性水平。滩涂草兔种群遗传多样性偏低可能是种群内部较强的基因流和数量急剧减少所致。由系统进化树表明,中国草兔不同地理种群并没有完全依据其地理来源形成明显的地理谱系结构,但在一定地域范围内可以作为进化显著单元的遗传独特种群。

To analyze the genetic diversity and phylogenetic evolution of the coastal wetland population of Lepus capensis, partial mitochondrial DNA control region was sequenced and mtDNA D - Loop segments of different geo- graphic populations of Lepus capensis in China were analyzed. The results showed that 4 polymorphic sites were de- tected by using the 566 bp segments of mitochondrial DNA control region from ten Lepus capensis individuals and three haplotypes were defined. The haplotype diversity and nucleotide diversity were 0. 689 and 0. 003 06 respec- tively, which showed a relative low genetic diversity level. The main reason for low genetic diversity may be the strong gene flow and dramatically reduced quantity within the population. Phylogenetic tree showed that no signifi- cant geological architecture was detected from different geological populations of Lepus capensis in China but these populations found in a certain area can be defined as unique genetic populations of evolutionarily significant units.