肠缺血再灌注过程中犬体内超氧化物歧化酶的改变

CHANGES IN SERUM SUPEROXIDE DISMUTASE ASSOCIATED WITH SMALL INTESTINAL ISCHEMIA AND REPERFUSION

本文实验采用FMT-182型γ免疫计数器,利用放射免疫法对4组不同时间肠缺血再灌注犬(对照组,绞窄2小时组,绞窄4小时组和绞窄6小时组)的血清进行检测,探讨机体自身超氧化物歧化酶(SOD)的改变。实验发现,肠绞窄2小时再灌注组其SOD变化呈上升趋势,而绞窄4小时和6小时再灌注组其SOD表现为下降。各组之间SOD改变有显著差异(P<0.05)。与对照组相比,绞窄4小时组和绞窄6小时组动物在术后4小时和8小时其SOD下降显著(P<0.05和P<0.01)且病理损害明显。由于SOD是氧自由基的清除剂,通过测定其活性可间接了解O_2^-等自由基对肠组织缺血病灶的影响,对判断预后有一定帮助。

Serum superoxide dismutase (SOD) were determined by FMT-182 γ radial immune counter. Serum obtained from dogs that were selected to have the intestinal ischemia and reperfusion with four different duration (Control group, Two-hour group, Four-hour group and Six-hour group). To determine witch change would occur in this procedure, we found that elevation of SOD occur in the Two-hour group and decrease in the Four and Six-hour groups. There are the significant difference among all groups (P<0.05). The significant decrease of SOD occur at 4 and 8 hours after isehemia in the Four and Six hour group as compared with Control group (P<0.05, P<0.01). SOD is one of the free radical scavengers, its change could prove helpful in evaluation bowel prognosis in cases of intestinal ischemia.