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RBP4诱导HUVEC炎症反应作用机制的研究 被引量：2

RBP4 Induced HUVEC Inflammatory Response Mechanism ofAction
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摘要 目的:探讨血清视黄醇结合蛋白4(RBP4)诱导人脐静脉内皮细胞(HUVEC)炎症的作用机制。方法:采用MTT还原法检测细胞活性,通过DCFH-DA检测活性氧的生成,Western blotting分析相关蛋白的表达,凝胶滞留法(EMSA)分析NF-kB的DNA结合活性。结果:RBP4处理后,MTT检测发现人脐静脉内皮细胞存活率显著低于对照组,流式细胞仪的结果表明活性氧的水平也显著升高。结论:RBP4可诱导人脐静脉内皮细胞(HUVEC)毒性及炎症反应。 Objective： To investigate the mechanism of action of serum retinol binding protein 4（RBP4） in human umbilical vein endothelial cells（HUVEC） induced inflammation.Methods： MTT reduction assay cell activity by DCFH-DA to detect reactive oxygen species generation,Western blotting analysis of related proteins,gel retardation（EMSA） analysis of NF-kB DNA binding activity.Results： RBP4 in treatment,MTT assay found that human umbilical vein endothelial cells was significantly lower than the control group,the flow cytometry results show that reactive oxygen species levels were also significantly elevated.Conclusion： RBP4 in human umbilical vein endothelial cells（HUVEC） could induce toxicity and inflammatory response.
作者 付鑫 张彦红 邹慕蔚
机构地区 沈阳医学院沈洲医院心血管二科
出处 《中国医药导刊》 2013年第3期524-525,共2页 Chinese Journal of Medicinal Guide
关键词 血清视黄醇结合蛋白4(RBP4) 人脐静脉内皮细胞(HUVEC) 作用机制 Serum retinol binding protein 4（RBP4） Human umbilical vein endothelial cells（HUVECs） Mechanism
分类号 R363 [医药卫生—病理学]
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  • 1Frey SK, Andrea H, Britta N, et al. Effect of renal replacement therapy on retinol-hinding protein 4 isoforms [J]. Clinica Chimica Acta, 2009,401 (1-2) : 46-50.
  • 2Jaconi S, Saurat JH, Siegenthaler G. Analysis of normal and truncated holo- and apo-retinol-binding protein (RBP) in human serum: altered ratios in chronic renal failure[J]. Eur J Endoerinol, 1996,134(5) : 576-582.
  • 3Graham TE, Berndt J, Kralisch S, et al. Serum retinol binding protein is more highly expressed in visceral than in subcutaneous adipose tissue and is a marker of intraabdominal fat mass[J]. Cell Metab, 2007,6 (1) : 79-87.
  • 4Bajzova M, Kovacikova M,Vitkova M,et al. Retinol-binding protein 4 expression in visceral and subcutaneous fat in human obesity[J]. Physiol Res, 2008,57(6): 927-934.
  • 5Graham TE, Yang Q, Bliaher M, et al. Retinol-binding protein4 and insulin resistance in lean,obese,and diabetic subjects[J]. N Engl J Med,2006,354(24) :2552-2563.
  • 6Kovacs P ;Geyer M, Berndt J, et al. Effects of genetic variation in the human retinol binding protein-4 gene (RBP4) on insulin resistance and fat depot-specific mRNA expression[J]. Diabetes, 2007,56 (12) : 3095-3100.
  • 7Yang Q,Graham TE, Mody N, et al. Serum retinol binding protein 4 contributes to insulin resistance in obesity and Type 2 diabetes[J]. Nature, 2005,436 (7049) :356- 362.
  • 8Cho YM, Youn BS, Lee H, et al. Plasma retinol-binding protein-4 concentrations are elevated in human subjects with impaired glucose tolerance and type 2 diabetes[J]. Diabetes Care, 2006,29 ( 11 ) : 2457-2461.
  • 9Jia W, Wu H, Bao Y, et al. Association of serum retinol binding protein 4 and visceral adiposity in Chinese sub- jects with and without type 2 diabetes[J]. J Clin Endocrinol Metab, 2007,92(8) : 3224-3229.
  • 10von Eynatten M, Lepper PM, Liu D,et al. Retinol-binding protein 4 is associated with components of the metabolic syndrome,but not with insulin resistance, in men with type 2 diabetes or coronary artery disease[J]. Diabetologia,2007,50(9) : 1930-1937.

共引文献15

同被引文献26

  • 1Chen Y,Azman SN,Kerishnan JP,et al.Identification of host-immune response protein candidates in the sera of humanoral squamous cell carcinoma patients[J].PLo S One,2014,9(10):e109012.
  • 2Pérez-Sayáns M,Somoza-Martín JM,Barros-Angueira F,et al.Genetic and molecular alterations associated with oral squamous cell cancer(Review)[J].Oncol Rep,2009,22(6):1277-1282.
  • 3Forastiere AA,Goepfert H,Maor M,et al.Concurrent chemotherapy and radiotherapy for organ preservation in advanced laryngeal cancer[J].N Engl J Med,2003,349(22):2091-2098.
  • 4Graham TE,Yang Q,Blüher M,et al.Retinol-binding protein 4 and insulin resistance in lean,obese,and diabetic subjects[J].N Engl J Med,2006,354(24):2552-2563.
  • 5Ost A,Danielsson A,Lidén M,et al.Retinol-binding protein-4attenuates insulin-induced phosphorylation of IRS1 and ERK1/2 in primary human adipocytes[J].FASEB J,2007,21(13):3696-3704.
  • 6Frey SK,Nagl B,Henze A,et al.Isoforms of retinol binding protein4(RBP4)are increased in chronic diseases of the kidney but not of the liver[J].Lipids Health Dis,2008,27(7):29.
  • 7Chaves GV,Peres WA,Gon?alves JC,et al.Vitamin A and retinolbinding protein deficiency among chronic liver disease patients[J].Nutrition,2015,31(5):664-668.
  • 8P Verner.Linear ion trap and its application in proteomic analysis[J].ChemickéListy,2005,99(12):937-942.
  • 9Cheng Y,Liu C,Zhang N,et al.Proteomics analysis for finding serum markers of ovarian cancer[J].Biomed Res Int,2014,2014:179040.
  • 10Abola MV,Thompson CL,Chen Z,et al.Serumlevels of retinolbinding protein 4 and risk of colon adenoma[J].Endocr Relat Cancer,2015,22(2):L1-4.

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中国医药导刊
2013年 第3期

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