DNA损伤检验点ATM-chk2通路影响衰老的作用机制分析

Mechanism analysis of DNA damage checkpoint ATM-chk2 path influence and function of delaying senility

目的分析DNA损伤检验点ATM-chk2通路对影响衰老的作用机制。方法选择SD大鼠4个月龄、12个月龄、20个月龄和28个月龄各10只,分别取4个月龄、12个月龄、20个月龄和28个月龄大鼠的骨髓组织进行ATM-chk2通路的观察,并对结果进行分析。结果 4个月龄、12个月龄、20个月龄和28个月龄SD大鼠的ATM基因表达随着月龄增加,呈逐渐下降趋势,不同月龄大鼠间比较,差异具有显著性(P<0.05);ATM-chk2通路基因蛋白表达呈逐渐上升趋势,组间比较差异具有显著性(P<0.05)。结论 ATM基因表达及ATM-chk2通路基因蛋白表达水平和大鼠月龄直接相关,年龄越大ATM基因表达日渐降低,ATM-chk2通路基因蛋白表达呈逐渐上升趋势。

Objective To analyze epimedium glycoside on DNA damage checkpoint ATM-chk2 channel effects and its mechanism of action of anti-aging. Methods SD rats choose 4 months and 12 months, and 20 months and 28 months each 10 only,and then select 4 months rats and only use epimedium glycoside feeding set to epimedium glycoside groups,namely take 4 months and 12 months,and 20 months and 28 months of rat bone marrow tissue for ATM-chk2 path of observation,then epimedium glycoside rats for feeding, respectively on 12 months and 20 months and 28 months,and when to take the bone marrow ATM-chk2 path and its gene protein observation, and the analysis of the results. Results 4 months and 12 months,and 20 months and 28 months SD rats ATM gene expression with months increase, had a gradual decline,different months between rats compared with significant difference（P 〈 0.05）;ATM-chk2 path gene protein expression was gradually increasing, was compared between group with significant difference （P 〈 0.05）;Epimedium glycoside rats in 12 months,20 months and 28 months respectively ATM gene expression than before epimedium glycoside feeding rats increased obviously,was compared between group with significant difference（P 〈 0.05）, ATM-chk2 path gene protein expression is gradually decline,is compared between group with significant difference （P 〈 0.05）. Conclusion The ATM gene expression and ATM-chk2 path phase protein gene expression level and rats is directly related to the age of the moon,epimedium glycoside can effectively improve SD rats ATM-chk2 path related protein gene expression level, at the same time reduce the ATM gene expression level,in order to delay the SD rats the purpose of aging.