响应曲面法优化平菇多糖提取工艺

Optimization of polysaccharides extraction process from Pleurotus ostreatus by response surface methodology

采用Box-Behnken设计的响应曲面法对平菇多糖的热水浸提工艺进行优化。在单因素试验的基础上,运用Design Expert 8.05b软件设计试验,选取料液比、提取温度和提取时间3个因素进行响应曲面分析,建立平菇多糖得率的二次多项数学模型。在分析各因素的显著性和交互作用后,得出平菇多糖热水浸提工艺的最佳条件为料液比1∶44.6(g∶mL),提取温度90℃,提取时间4.3h,平菇多糖的得率为3.25%。

Response surface methodology by Box-Behnken was applied to optimize the hot water extraction process of polysaccharides from P/eurotus ostreatus （POP）. On the basis of one-factor experiments, the Design Expert 8.05b software was used to design the experiments and the response surface analysis of 3 factors was adopted. A second order quadratic equation for extraction of polysaccharides was built. After analyzed the significance of various factors and interactions, the optimal extraction conditions of the POP were concluded as follows： the ratio of material to water was 1： 44.6（g/ml）, extraction temperature was 90℃, and extraction time was 4.3h. Under the optimal conditions, the yield of POP reached 3.25%.