骨髓间充质干细胞移植心肌梗死大鼠的心功能

Cardiac function of myocardial infarction rats after bone marrow mesenchymal stem cell transplantation

背景:前期研究发现移植的骨髓间充质干细胞能在受损心肌组织内存活和分化。目的:进一步观察局部注射移植同种异体骨髓间充质干细胞对心肌梗死模型大鼠心功能的影响。方法:体外培养的同种异体骨髓间充质干细胞达到一定数量(106)后用BrdU标记。24只SD大鼠随机分为3组:正常对照组未行冠状动脉前降支结扎,取骨髓间充质干细胞约1mL直接注射到冠状动脉前降支心肌的周围;假移植组:在冠状动脉前降支结扎后7d,取等量DMEM培养液直接注射到梗死心肌的周围;骨髓间充质干细胞移植组:冠状动脉前降支结扎后7d,取等量骨髓间充质干细胞直接注射到梗死心肌的周围;分别于移植前、移植后5周测定大鼠心功能。结果与结论:①移植后5周假移植组最大左室收缩末压、左室内压最大(最小)变化速率均低于移植前(P<0.01),而左室舒张末压高于移植前(P<0.01);移植后5周骨髓间充质干细胞移植组最大左室收缩末压、左室内压最大(最小)变化速率高于移植前(P<0.01),而左室舒张末压低于移植前(P<0.01)。②移植后5周,骨髓间充质干细胞移植组大鼠的最大左室收缩末压、左室内压最大(最小)变化速率均高于假移植组(P<0.01),而左室舒张末压明显低于假移植组(P<0.01)。结果可见骨髓间充质干细胞移植可明显改善心肌梗死模型大鼠心功能。

BACKGROUND： Previous studies have shown that the transplanted bone marrow mesenchymal stem cells can survive and differentiate in the damaged myocardial tissue. OBJECTIVE： To observe the effect of local injection and transplantation of allogeneic bone marrow mesenchymal stem cells on cardiac function in rat model of myocardial infarction. METHODS： The allogeneic bone marrow mesenchymal stem cells were cultured in vitro and then labeledwith BrdU after the number of the cells reached to a certain amount （106）. A total of 24 Sprague-Dawley rats were randomly divided into three groups. Rats in the normal control group did not undergo the anterior descending coronary artery ligation, and then 1 mL bone marrow mesenchymal stem cells were injected into the myocardium surrounding the anterior descending coronary artery; in the sham-transplantation group, 1 mL dulbecco’s modified eagle’s medium was injected into the surrounding infarcted myocardium at 7 days after anterior descending coronary artery ligation; in the transplantation group, bone marrow msenchymal stem cells in the same dose were injected into the surrounding infarcted myocardium at 7 days after anterior descending coronary artery ligation. The cardiac function of the rats was measured before transplantation and 5 days after transplantation. RESULTS AND CONCLUSION： The maximum left ventricular end-systolic pressure and maximum and minimum left ventricular pressure rising and dropping rates in the sham-transplantation group at 5 weeks after transplantation were lower than those before transplantation （P＆lt;0.01）, while the left ventricular end-diastolic pressure was higher than that before transplantation （P＆lt;0.01）; the maximum left ventricular end-systolic pressure and maximum and minimum left ventricular pressure rising and dropping rates in the transplantation group at 5 weeks after transplantation were higher than those before transplantation （P＆lt;0.01）, and the left ventricular end-diastolic pressure was lower than that before transplantation （P＆lt;0.01）. At 5 weeks after transplantation, the maximum left ventricular end-systolic pressure and maximum and minimum left ventricular pressure rising and dropping rates in the transplantation group were higher than those in the sham-transplantation group （P＆lt;0.01）, while the left ventricular end-diastolic pressure in the transplantation group was lower than that in the sham-transplantation group （P＆lt;0.01）. The results showed that bone marrow mesenchymal stem cell transplantation can significantly improve the cardiac function of a rat model of myocardial infarction.