依达拉奉联合神经干细胞移植修复大鼠脊髓损伤

Edaravone combined with neural stem cell transplantation for treatment of spinal cord injury in rats

背景:依达拉奉是一种自由基清除药,可以减轻受损神经组织水肿和改善脊髓损伤区微环境。目的:观察依达拉奉联合神经干细胞移植对大鼠脊髓全横断损伤的修复效果。方法:成年雌性SD大鼠80只,建立胸9脊髓全横断损伤模型,随机分为4组:对照组不做处理;依达拉奉组脊髓损伤后6h经尾静脉注射依达拉奉;神经干细胞移植组脊髓损伤后6h脊髓损伤区域注入神经干细胞悬液;依达拉奉+细胞移植组脊髓损伤后6h神经干细胞移植的同时尾静脉注射依达拉奉。结果与结论:造模后8周可观察到PKH-26标记的神经干细胞在体内存活并在脊髓内迁移;细胞移植组和依达拉奉联+细胞移植组可见少量连续性神经纤维通过损伤区。荧光金逆行脊髓追踪显示神经干细胞移植组和依达拉奉+细胞移植组可见被荧光金标记的神经锥体细胞穿越损伤区。PKH-26标记的阳性细胞数及荧光金阳性神经纤维数:依达拉奉+细胞移植组最多,依达拉奉组、神经干细胞移植组次之,对照组最少,各组之间差异有显著性意义(P<0.05);后肢功能运动BBB评分依次为依达拉奉+细胞移植组>神经干细胞移植组>依达拉奉组>对照组。提示依达拉奉能促进神经干细胞在损伤区的存活并向神经细胞分化,依达拉奉联合神经干细胞移植有促进细胞移植修复大鼠脊髓损伤的效果。

BACKGROUND： Edaravone is a kind of free radical scavenging drug, which can reduce the edema of the damaged nerve tissue and improve the microenvironment of spinal cord injury areas.OBJECTIVE： To observe the effects of edaravone combined with neural stem cell transplantation in the treatment of spinal cord injury in rats. METHODS： A total of 80 Sprague-Dawley rats were used to establish the T9 spinal cord transaction models, and the models were randomly divided into four groups （n=20）, namely the control group, edaravone group, neural stem cells transplantation group and neural stem cell transplantation ＋ edaravone group. The models in the control group received no treatment; models in the edaravone group were injected with edaravone via tail vein at 6 hours after spinal cord injury; models in the neural stem cell transplantation group were injected with neural stem cell suspension in the spinal cord injury area at 6 hours after spinal cord injury; models in the neural stem cell transplantation ＋ edaravone group were injected with neural stem cells and edaravone via tail vein at 6 hours after spinal cord injury. RESULTS AND CONCLUSION： PKH-26-labeled neural stem cells survived and migrated toward the spinal cord at 8 weeks postoperatively; a small amount of continous nerve fibers migrated across the injury site in the neural stem cell transplantation group and neural stem cell transplantation ＋ edaravone group. Fluorogold retrograde spinal cord tracing showed the fluorogold-labeled pyramidal cells migrated across the injury site in the neural stem cell transplantation group and neural stem cell transplantation ＋ edaravone group. The number of fluorogold- labeled nerve fibers and PKH-26 positive cells was largest in the neural stem cell transplantation ＋ edaravone group, greater in the edaravone group but little in the control group, and there was significant difference between groups （P＆lt;0.05）. The BBB scores of the hind limb motor function： neural stem cells transplantation ＋ edaravone group＆gt;neural stem cell transplantation group＆gt;edaravone group＆gt;control group. Edaravone can promote the survival of neural stem cells and their differentiation into neural cells at the injury site. Edaravone combined with neural stem cell transplantation can promote the repair of spinal cord injury in rats.