Cyclin D1在中耳胆脂瘤中的表达及意义

Expression and significance of CyclinD1 in middle ear cholesteatoma

目的检测细胞周期蛋白D1(Cyclin D1)在中耳胆脂瘤中的表达,探讨其在胆脂瘤发病机制中的作用。方法采用免疫组织化学技术PV-6000二步法检测CyclinD1在30例中耳胆脂瘤组织及15例正常耳后皮肤中的表达。结果 CyclinD1在中耳胆脂瘤组织及正常耳后皮肤中的阳性率分别为70.0%、26.7%,两者比较差异有统计学意义(χ2=5.951,P<0.05)。结论 CyclinD1在中耳胆脂瘤上皮中存在高表达,这反映了胆脂瘤上皮的过度增殖性,说明CyclinD1可能在中耳胆脂瘤的发生、发展中起重要作用。

Objective To investigate the expression of CyclinD1 protein and its role in the occurrence and development of middle ear cholesteatoma. Methods Immunohistochemical technology（PV-6000） was applied to examine the expres- sion of CyclinD1 in 30 cases of cholesteatoma tissues and 15 cases of normal auricle skin. Result The positive expression rate of CyclinDl protein in the middle ear cholesteatoma tissues was 70.0% and 26.7% in normal auricle skin（x2 = 5.951 ,P 〈 0.05）. Conclusion The overexpression of CyclinD1 suggested the hyperproliferative nature of cholesteatoma epithelium. CyclinD1 may play an important role in the formation and development of middle ear cholesteatoma.