力竭运动对小鼠骨骼肌氧化应激和DNA损伤的影响

Effect of exhausted exercise on oxidative stress and DNA damage of skeletal muscle cells

目的:研究力竭运动对小鼠骨骼肌氧化应激和DNA损伤的影响,探讨骨骼肌细胞DNA损伤的氧化损伤机制,进一步揭示运动性疲劳的损伤机理;方法:采用单细胞凝胶电泳对力竭运动小鼠不同恢复期骨骼肌细胞的DNA损伤效应进行测定;结果:小鼠骨骼肌细胞在大强度力竭运动后即刻和24 h的DNA损伤显著高于对照组损伤水平(P<0.05),其中即刻组的DNA损伤程度最为显著(P<0.01),而运动后48 h彗星各项指标与对照组的差异无统计学意义(P>0.05);结论:力竭运动所致的骨骼肌细胞DNA损伤与氧化应激水平有密切的关系,运动性氧应激参与介导了细胞DNA损伤,运动性氧应激是组织细胞DNA损伤的机制之一,适时有效的抗氧化剂补充将有利于防护组织细胞DNA损伤。

Objective：To study the effect of exhaustive exercise on oxidative stress and DNA damage of skeletal muscle cells, DNA injury mechanism of oxidative damage, and further reveal the exercise-induced fatigue damage mechanisim. Methods： Using the single cell gel electrophoresis to measure the DNA damage effect of exhaustive exercise on skeletal muscle cell of mice. Results：Mice skeletal muscle cell in high -intensity exhaustive exercise immediately after injury and 24 h DNA were higher than those in control group （ P 〈 0.05 ）, of which DNA damage immediately alter exercise is the most significant （ P 〈 0.01 ） and 48 h after exercise comet indicators had no significant difference （ P 〉 0. 05 ） with control group; Conclusion： exilaustive exercise induced skeletal muscle cell DNA damage and oxidative stress levels are closely related, exercise -induced oxidative stress involvement mediates cell DNA damage, movement of oxygen stress is one of the mechanisms of cell DNA damage, timely and effective antioxidant supplementation will be helpful for protective tissue cell DNA damage.