SMU1对正常人体外PBMNC功能效应的影响

STUDIES OF THE EFFECTS OF SMU1 ON NORMAL PBMNC IN VITRO

目的 :研究SMU1(自制抗CD3)对正常人外周血单个核细胞 (PBMNC)的体外功能效应。方法 :①以SMU16种不同浓度、IL 2、MCD3(丝裂性抗CD3)孵育PBMNC ,观察细胞密度和形态变化 ,MTT法测定细胞增殖力 ;②分别用SMU10 .5mg/L、5mg/L、IL 2 2 0 0kU/L培养PBMNC 3d ,ELISA法测定上清液IL 6、IL 8、IL 12、IFN α、TNF α及G CSF水平。结果 :IL 2组和MCD3组细胞增多成团 ,细胞异形性明显 ,增殖力显著高于实验对照组。而SMU1各组细胞形态、密度无明显变化。PBMNC经SMU1孵育后上清IL 8、IL 12、IFN α水平显著升高 ,经IL 2孵育后上清IL 8、TNF α、IFN α水平升高。结论 :SMU1无丝裂作用 ,但对PBM NC分泌细胞因子有重要影响 ,其促分泌作用与IL

Objective:To study the in vitro effects of SMU1 (a self made monoclonal antibody to CD3)on normal PBMNCs. Methods:①To culture normal PBMNCs with 6 concentration gradients of SMU1、interleukin 2、MCD3(a mitogenic anti CD3),observe the cells' quantitative and morphologic changes,and assay cells' proliferation with MTT method.②To culture PBMNCs with SMU1(0.5mg/L or 5mg/L)or IL 2(200kU/L) for 3 days, collect the supernates,and assay IL 6,IL 8,IL 12,IFN α and TNF α levels with ELISA. Results:Cells' quatitative incresase and morphologic change were observed in groups of IL 2 and MCD3, wheras the cells remained their primary number and morphology in the SMU1 groups and control group. Proliferative tests showed that both IL 2 and MCD3 significantly promote cells to proliferate, with MCD3 the stronger. SMU1 induced production of IL 8,IL 12 and IFN α, and IL 2 induced secretion of IL 8,TNF α and IFN α Secretogoge action of SMU1 was correlated with its dose. Conclusion:SMU1 is probably non mitogenic anti CD3,but has great influence on cytokine production. As regards secretogoge action, there is difference between SMU1 and IL 2.