人脑胶质瘤PTEN表达的意义

Clinical significance of PTEN expression in human glioma

目的 检测 PTEN蛋白在人脑胶质瘤细胞中的表达 ,探讨 PTEN表达与胶质瘤病理分级和预后的关系 .方法 应用免疫组化方法 ,检测 6 3例人脑胶质瘤标本中的 PTEN表达 ,分析其与胶质瘤细胞恶性程度与预后的关系 .结果 PTEN阳性染色颗粒定位于细胞质中 ,在 6 3例人脑胶质瘤标本中 ,有 38例 (6 0 % ) PTEN呈阳性表达 ;低分化组的阳性表达率 ( 和 级为 37% ,11/ 30 )明显低于高分化组的阳性表达率 ( 和 级为 82 % ,2 7/ 33) ,两者之间差异显著 (P<0 .0 1) . PTEN表达阳性与阴性患者在 1或 3 a的存活率比较中 ,在 和 级胶质瘤患者中无显著性差异 (P>0 .0 5 ) ;在 级和 级患者中 ,3a的存活率比较差异显著 ,PTEN表达阳性者较阴性者的存活率高 (P<0 .0 5 ) ;PTEN阳性表达的患者中 ,高分化组 ( 和 级 )和低分化组 ( 和 级 )之间在 1或3a存活率比较差异显著 (1a存活率比较 ,P<0 .0 5 ;3a存活率比较 ,P<0 .0 5 ) .结论 PTEN基因突变或缺失在人脑胶质瘤的发生发展中起重要作用 ,与肿瘤恶性分化程度密切相关 ,也与患者预后相关 。

AIM To study phosphatase and tensin homology deleted on chromosome ten (PTEN) protein expression andthe relationship between its expression and the pathological grade or prognosis of patients with glioma. METHODS Sixty three cases of glioma specimens with follow up history were examined by immunohistochemistry. The relationship between PTEN expression and pathological grade or prognosis of patients with glioma was investigated. RESULTS The PTEN positive staining was found in the plasma. The positve rate was 60% (38/63), and the positive rates decreased with progression of pathological grade ( P <0.01), regardless of age and sex. In grade Ⅲ and Ⅳ cases, there was a remarkable difference in one year or three year survival rate; the survival rate of PTEN positive cases was higher than that of negative cases. In PTEN positive cases, the difference of one year or three year survival rates existed between Ⅰ,Ⅱ and Ⅲ,Ⅳ( P <0.05, respectively). CONCLUSION PTEN gene mutation and deletion play a key role in the glioma genesis and development. It is considered a valuable factor in evaluating the glioma malignancy and the prognosis of the patients.