IL-12对哮喘患者Th_1/Th_2的调节

IL-12 affects Th_1/Th_2 cells of patients with asthma

目的 探讨白细胞介素 12 (IL- 12 )对哮喘患者辅助性 T细胞亚群的调节作用 .方法 收集 14例支气管哮喘患者 ,10例正常对照者的外周血单个核细胞 (PBMC) ,加入不同浓度 IL- 12进行体外培养 ,收集细胞培养上清液 ,以酶联免疫吸附法测定 IL - 4及 IFN-γ.并用酶联免疫斑点法(EL ISPOT)检测哮喘患者体外培养的 PBMC中的 Th1 和Th2 阳性率 .结果 哮喘患者 PBMC培养上清液中 IFN-γ产生减少 (P<0 .0 1) ,IL- 4产生增高 (P<0 .0 1) .IL- 12对 IFN-γ的产生有刺激作用 (P<0 .0 1) .较高浓度 IL - 12 (5μg·L- 1 )刺激 PBMC产生 IL - 4(P<0 .0 1) ,低浓度 IL - 12 (0 .0 5μg· L- 1 )抑制 IL- 4的产生 (P<0 .0 1) .EL ISPOT检测出的结果是 ,哮喘患者 PBMC中 Th1 阳性率与正常对照组无显著差别 (P>0 .0 5 ) ,而 Th2 阳性率明显增高 (P<0 .0 1) .不同浓度 IL- 12均能显著增高 Th1 的阳性率 (P<0 .0 1) ,较高浓度IL - 12亦能增高 Th2 阳性率 (P<0 .0 5 ) ,低浓度 IL - 12降低Th2 阳性率 (P<0 .0 1) .结论 IL- 12参与了哮喘的发病过程 ,它通过调节 Th1 / Th2

AIM To study the effect of IL 12 on Th cells of patients with asthma. METHODS We studied the effect of IL 12 on cultured PBMC obtained from 14 patients with asthma and 10 normal subjects. ELISA was used to determine the concentration of IL 4 and IFN γ. Enzyme linked immunospot assay (ELISPOT) was used to detect percentages of Th 1 and Th 2 cells in PBMC from 14 patients with asthma and 10 normal subjects. RESULTS IFN γ levels were much lower ( P <0.01) and IL 4 levels much higher ( P <0.01) than normal subjects in supernatant of PBMC. IL 12 significantly increased IFN γ secret ( P <0.01) by PBMC of patients with asthma. High dose of IL 12 (5 μg·L -1 ) also increased IL 4 secret ( P <0.05), but low dose of IL 12 (0.05 μg·L -1 ) significantly inhibited IL 4 secret ( P <0.01). There was no difference of mean percentage of Th 1 cells of PBMC in patients with asthma from normal subjects ( P >0.05). The mean percentage of Th 2 cells was much higher in asthma ( P <0.01). IL 12 Could significantly increase Th 1 percentage in PBMC from asthma patients ( P <0.01). High concentration of IL 12 also increased Th 2 percentage in PBMC ( P <0.05), but lower concentration of IL 12 significantly decreased Th 2 percentage ( P <0.01). CONCLUSION IL 12 is involved in the asthma process and plays an important role in affecting Th 1/Th 2 cells activity and cytokine production.