斑点金免疫渗滤试验检测结核血清抗体方法的建立及初步临床应用

Detection of Serum Antibody of Tuberculosis with Dot Immunogold Filtration Assay: Establishment of the Method and Preliminary Clinical Application

从结核分枝杆菌 (H37Rv菌株 )中提纯了阿拉伯糖甘露糖脂 (LAM)抗原 ,将其用于斑点金免疫渗滤试验以检测结核血清抗体。对痰菌阳性组和痰菌阴性组的活动性肺结核病人血清标本 ,本法的阳性率分别为 91 9% (6 8/ 74)和 5 3 3 % (8/ 15 )。对正常人、肺癌病人和肺炎病人血清标本 ,本法的阴性率分别为 96 4% (80 / 83)、 94 4% (17/ 18)和 10 0 0 % (12 / 12 )。

The lipoarabinomannan (LAM) antigen was purified from Mycobacterium tuberculosis strain H37Rv and was used in dot immunogold filtration assay to detect serum antibodies of patients with tuberculosis.Of serum samples from active pulmonary tuberculosis patients,the positive rates of this assay for sputum positive and sputum negative groups were 91 9%(68/74) and 53 3%(8/15) respectively, and of those from healthy people,lung cancer patients and pneumonia patients,the negative rates of this assay were 96 4%(80/83),94 4%(17/18) and 100 0%(12/12) respectively