P16蛋白和视网膜母细胞瘤蛋白在106例患者肺癌组织中的表达

The expression of P16 and Rb proteins in 106 cases of lung cancer

目的 探讨P16和视网膜母细胞瘤 (Rb)蛋白表达与肺癌细胞增殖的关系及其临床意义。方法 应用免疫组化技术检测了 6 6例非小细胞肺癌 (NSCLC)和 40例小细胞肺癌 (SCLC)患者肺癌组织中P16蛋白和Rb蛋白表达。结果 NSCLC中P16蛋白与Rb蛋白的表达呈显著负相关 (χ2 =10 5 2 ,P <0 0 1)。P16蛋白或Rb蛋白的表达与肺癌分期、分化程度、病理分型、淋巴结转移无明显相关 (P >0 0 5 )。SCLC中Rb蛋白表达的缺失率 (78% )与NSCLC(2 1% )比较 ,差异有显著性 (χ2 =32 3,P <0 0 1)。结论 Rb蛋白在SCLC中的高缺失率使之可能成为SCLC诊断的重要参考指标。在NSCLC中P16蛋白与Rb蛋白的表达呈负相关 ,进一步证实了在G1期调节过程中P16与Rb之间存在着负反馈环路。

Objective To observe the relationship between the expression of P16 and Rb proteins and the proliferation of lung cancer cells.Methods The expressions of P16 and Rb proteins were measured in 66 cases of NSCLC and 40 cases of SCLC by means of immunohistochemistry technique. Results The inverse correlation of Rb and P16 proteins expression was confirmed in 66 cases of NSCLC (χ 2=10.52, P <0.01). No relationship between the expression of P16 and differentiation, stage, lymph node metastasis or pathological type was found ( P >0.05). There was no correlation between the expression of Rb and differentiation, stage, lymph node metastasis or pathological pattern ( P >0.05). The frequency of the loss of Rb protein expression in SCLC (78%) was significantly higher than in NSCLC (21%, χ 2=32 3, P <0 01) Conclusions Loss of Rb protein expression in SCLC may be an important marker of the diagnosis of SCLC. The expression of P16 adversely correlates with that of Rb. This study provides strong confirmation about that there might be a negative feedback between P16 and Rb in phase G 1.