EGb761对抗海人藻酸神经毒性的作用

PARTIAL PROTECTIVE EFFECTS OF EGB761 AGAINST KAINATE NEUROTOXICITY

目的 :探讨银杏叶提取物 ( EGb761)对海人藻酸 ( KA)引起皮层神经元毒性作用的影响及可能的作用机制。方法 :在小鼠原代培养的皮层神经元 KA毒性模型上 ,采用形态学观察 ,MTT比色分析 ,ELISA及单细胞内游离钙离子浓度测定等方法。结果 :EGb761( 62 .5 μg/ml)及内酯 B( 3 7.5 μg/ml)预处理 2 4h可不同程度地对抗 KA( 0 .5 mmol/L)短时间作用引起神经元损伤。结论 :EGb761可部分对抗海人藻酸对培养神经元的毒性作用 ,其作用机制可能与 EGb761及其活性成分内酯 B维持细胞内钙离子稳态有关。

Objective:To determine whether the extract of leaves of Ginkgo biloba L ( EGb761) have protective effects against kainate neurotoxicity and what is the possible mechanism.Methods: Morphological observation, MTT staining, ELISA and the intracellular free calcium concentration of single neuron determination were used on kainate neurotoxicity of primary cultures from mouse cerebral cortex. Results: Pretreatment with EGb761(62.5μg/ml)and ginkgolide B (37.5μg/ml)for 24h protected the neurons against KA (0.5 mmol/L)-induced injury in different degree.Conclusions: EGb761 and its active component ginkgolide B partially prevents neurons from kainate neurotoxicity through maintaining the intracellular calcium homeostasis.[